中文摘要：

对分离自健康未断奶仔猪粪便的抗猪链球菌的戊糖乳杆菌LPL1—5所产细菌素进行提取、纯化，以及理化和生物学特性研究。戊糖乳杆菌LPL15所产细菌素经饱和度为80％的硫酸铵盐析、葡聚糖凝胶树脂G-10层析和SPSepharoseFastFlow阳离子树脂交换层析可得到纯化的细菌素，其比活力为1732．16AU／mg，纯度较发酵液提高了26．37倍；Tricine—SDS-PAGE凝胶电泳及活性检测试验结果显示，3313～5856u范围内存在1条活性蛋白带；纯化后细菌素特性分析发现，该细菌素具有良好的热稳定性、酸耐受性及溶解性，并基本确定其等电点为8．7；胃蛋白酶、胰蛋白酶、中性蛋白酶和蛋白酶K处理可使细菌素完全失活；该细菌素抑菌谱较广，不仅对猪链球菌（Sreptococcussuis）、单核细胞增生李斯特氏菌（Listeriamonocytogenes）、金黄色葡萄球菌（sfnphylococcus aereu）等革兰氏阳性菌有明显抑制作用，同时对大肠杆菌（Escherichiacoli）及假单胞菌（Pseudomonasspp．）等革兰氏阴性菌也表现出抑菌活性，具有作为食品防腐剂良好的应用前景。

英文摘要：

Purification and characterization of an anti-streptococcus suis bacteriocin produced by Lactobacillus pentosus LPL1-5,isolated from faeces of healthy piglets,were studied. The bacteriocin was purified by 80% ammonium sulphate precipitation, Sephadex G-10 gel filtration chromatography and SP Sepharose Fast Flow cation exchange chromatography. And the specific activity of purified bacteriocin reached 1 732.16 AU/mg and the purity was increased by 26.37 fold. Tricine- SDS-PAGE of the purified bacteriocin contained only one active protein band between 3.3 and 5.8 ku. Purified bacteriocin was heat resistant, active at pH values between 2.0 - 8.0 and inactivated by pepsin, trypsin,neutro-proteinase and proteinase K. It also could easily be dissorved in different media and its isoelectric point was 8.7. In addition, the bacteriocin had a broad inhibitory spectrum. It not only had a strong antimicrobial effect on gram-positive,such as streptococcus suis, Listeria monocytogenes, Staphylococcus aereu and etc, but also could inhibited several gram-negative bacteria, such as Escherichia coil, Pseudomonas spp and etc. These results indicated that the bacteriocin has a potential application as food additives and bio-preservatives in food industry.