中文摘要：

目的 探讨外周血T细胞受体（TCR）β链V区（BV）CDR3区谱型差异在慢性HBV感染者炎症发生中的作用。方法研究对象为9例慢性HBV感染无症状携带（AsC）者（AsC组）和12例活动期慢性乙型病毒性肝炎（CHB）患者（CHB组），分别采集其外周血，肝素钠抗凝，分离单个核细胞，提取RNA并应用RT-PCR扩增TCR BV的CDR3区基因24个家族，采用免疫指纹技术进行TCR BV各家族基因扫描和谱型分析。结果 两组外周血淋巴细胞TCR BV谱型均发生不同程度单克隆性、寡克隆性及偏峰性克隆增生，CHB组发生谱型偏移的TCR BV家族个数及CDR3谱型发生单克隆性和寡克隆性偏移家 族总数均显著多于AsC组（P〈0.05）。结论 CHB患者外周血T细胞克隆性增生程度高于AsC患者，此可能与慢性HBV感染者炎症的发生机制相关。

英文摘要：

To investigate the roles of diffference of T cell receptor （TCR） β chain variable region gene （BV） GDR3 sequences in the inflammation occurrence of patients infected HBV. Methods PBMCs were isolated from anticogulated blood of 9 immune tolerant chronic asymptomatic HBV carrier（AsC） （AsC group）and 12 chronic hepatitis B （CHB） patients（ CHB group）, and RT-PCR was proformed to amplificate 24 TCR BV families transcripts, and PCR products were genescaned by polyacrylamide gel electrophoresis, and the spectrum patterns were analyzed. Results Both groups showed T cell clonal expansion with various degree, and three perturbation patterns of T cell expansion were showed in their BV families, including monoclonicity, oligeclonicity and skewed peak patterns. The number Of monoclonicity and ohgeclonicitytotal perturbation families in CHB group was more than that in AsC group （ P 〈 0.05）. Conclusion CHB patients may have higher degree of T cell expansion than AsC, and this maybe involved in immunopathogensis mechanism of hepatitis B virus.