中文摘要：

目的建立HPLC波长切换法,对主产区与道地产区牡丹皮中没食子酸、氧化芍药苷、芍药苷、丹皮酚的含有量进行测定。方法牡丹皮提取液的分析采用Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）;甲醇（A）-0.05%磷酸水溶液（B）为流动相,梯度洗脱;体积流量1.0 m L/min;检测波长为270 nm（0~15 min,没食子酸）、230 nm（15~40 min,氧化芍药苷和芍药苷）、274 nm（40~70 min,丹皮酚）。结果没食子酸、氧化芍药苷、芍药苷、丹皮酚分别在0.010~0.300μg（r=0.999 7）、0.149~4.460μg（r=0.999 9）、0.088~2.640μg（r=0.999 9）、0.246~7.380μg（r=0.999 9）范围内与峰面积均呈良好的线性关系,加样回收率（n=6）分别为99.3%、98.9%、97.6%、102.9%,RSD分别为0.7%、0.8%、1.1%、0.6%。结论两个产区牡丹皮的质量相当,在亳州（主产区）引种该药材可行。

英文摘要：

AIM To establish a method for the determination of gallic acid,oxypaeoniflorin,paeoniflorin and paeonol in Moutan Cortex from main and genuine growing areas by HPLC with UV switch. METHODS The analysis of Moutan Cortex extract was carried out on Zorbax SB-C18column（ 4. 6 mm × 250 mm,5 μm）,mobile phase was methanol（ A）-0. 05% phosphoric acid aqueous solution（ B） with gradient elution,flow rate was 1. 0m L / min,and detection wavelength was set at 270 nm（ 0- 15 min,gallic acid）,230 nm（ 15- 40 min,oxypaeoniflorin and paeoniflorin） and 274 nm（ 40- 70 min,paeonol）. RESULTS The good linearities were in the ranges of 0. 010- 0. 300 μg（ r = 0. 999 7） for gallic acid,0. 149- 4. 460 μg（ r = 0. 999 9） for oxypaeoniflorin,0. 088- 2. 640 μg（ r = 0. 999 9） for paeoniflorin,and 0. 246- 7. 380 μg（ r = 0. 999 9） for paeonol,respectively. Average recoveries（ n = 6） were 99. 3%,98. 9%,97. 6% and 102. 9% with the RSDs of 0. 7%,0. 8%,1. 1% and 0. 6%,respectively. CONCLUSION The qualities of Moutan Cortex from two growing areas are similar,so the introduction of this plant to Bozhou（ main growing area） is feasible.