中文摘要：

目的：优选山茱萸的水提醇沉多糖提取工艺条件。方法：以山茱萸多糖含量为指标,蒽酮比色法测定指标成分含量,在单因素试验基础上,采用L9（3^4）正交试验考察料液比、超声提取时间、重复提取次数和乙醇浓度对山茱萸多糖含量的影响,优化其提取工艺。并用DPPH自由基清除率法和总抗氧化实验对其抗氧化活性进行测定。结果：山茱萸多糖最佳提取的工艺条件为料液比1∶10,提取时间20 min,重复提取次数1次,提取乙醇浓度60%。实验显示,山茱萸多糖具有浓度相关的自由基清除能力和抗氧化能力。结论：优选的水提醇沉提取工艺稳定可行,所提取山茱萸多糖具有较强的自由基清除效果,是一种极具开发潜力的优良天然抗氧化剂。

英文摘要：

Objective： To optimize the technology conditions of water extraction- alcohol precipitation for Cornus officinalis polysaccharides. Methods： With the content of Cornus officinalis polysaccharides as index tested by Anthrone colorimetry,based on the single factor test,effects of solid- liquid ratios,extraction time,repeat times and ethanol concentration were investigated by L9（ 34） orthogonal experiment. The antioxidant activities were determined by the total antioxidant activity DPPH free radical scavenging experiment. Results： The research indicated that the optimum condition for extraction of Cornus officinalis polysaccharides was solid- liquid ratio 1： 10,extraction time 20 min,repeated 1 time,ethanol dose 60%. The ABTS free radical scavenging experiments showed that Cornus officinalis polysaccharides demonstrated the dose- dependent free radical scavenging and antioxidant activates. Conclusion： This optimized technology was stable and feasible. The isolated Cornus officinalis polysaccharides demonstrated great free radical scavenging activities,and thus providing a potential natural antioxidant for further development.