中文摘要：

为寻找新的抗植物病原菌微生物资源,以苦槛蓝为对象开展了研究.从其叶、叶脉、茎、树皮、根共分离到104株内生真菌.经形态学初步鉴定,它们主要属于无孢类群、盘长孢属、青霉属、枝孢霉属和曲霉属.生物活性测试表明,所分离到的苦槛蓝内生真菌中有77.9％对包括小麦赤霉、大豆疫霉、香蕉炭疽和柑橘青霉中至少1种植物病原菌显示抑制活性,42.3％和11.5％的菌株对上述植物病原菌中的至少1种分别达到了中度抗菌和强烈抗菌.高效液相测试显示苦槛蓝提取物与5种具有强烈抗菌活性的苦槛蓝内生真菌提取物的谱图差异显著,表明这些内生真菌产生了不同于原植物代谢产物的新的抗植物病原菌活性物质.

英文摘要：

To search for new microorganisms with inhibitory activity against plant pathogens, Myoporum bontioides A. Gray was chosen to be studied. One hundred and four endophytic fungal cultures were isolated from the leaf , leaf vein, stem, bark, and root samples of Myoporum bontioides A. Gray. They mainly belonged to the genera of sterile mycelium, Gloeosporium spp., PeniciUium spp., Cladosporium spp. and Aspergillus spp. Primary bioassay of their metabolites showed that about 77.9% strains had inhibitory activity against at least one plant pathogens among Fusarium graminearum, Gloeosporium musae, Penicillium italicum and Phytophthora sojae （Kaufmann ＆ Gerdemann）. And the proportions of the strains with medium and high activity were ab out 42.3% and 11.5%, respectively. The HPLC analysis showed that the spectral profiles of the extracts of Myoporum bontioides A. Gray and five selective endophytic fungi with high antifungal activity were remarkably different, which suggested that these fungi produced different antifungal compounds in contrast to their mother plant.