中文摘要：

报道了水解酶催化二乙基锌与芳香醛的加成反应, 对反应条件进行了优化. 在最适条件（嗜热酯酶APE1547为酶源, 反应温度40℃ 氯仿为溶剂, 4-Cl-苯甲醛为底物）下, 加成反应生成的光学活性醇产率最高达78%, ee值最高可达56%. 通过实验结果和分子动力学分析对可能的反应机理进行了推测. 本研究进一步拓展了酶的非专一性.

英文摘要：

A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes is reported. Optimum conditions were screened out. Under the optimum conditions （i.e., reaction at 40 ℃ using hyperthermophilice esterase APE1547, 4-Cl-benzaldehyde substrate, and trichloromethane solvent）, this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%. The possible mechanism was proposed based on experimental observations and molecular dynamics simulation. These observations extend the phenomenon of enzyme promiscuity.