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中文摘要：
利用帆布固定化嗜热酯酶APE1547（3）在非水介质中催化转酯化反应进行酶促拆分（R,S）-2-甲基-1-丁醇制备（S）-2-甲基-1-丁醇（4）。初步探讨了溶剂、酰基供体、温度及底物配比对3的催化活力（EA3）与4对映选择性比率（E4）的影响。在最佳拆分条件下,EA3=0.53μmol.min-1.mg-1,E4=15.4。3套用8次后仍然保持较高的催化活性。
英文摘要：
（R,S）-2-methyl-1-butanol was resolved to give（S）-2-methyl-1-butanol（4） by transesterification using immobilization thermophilic esterase APE1547（3） on canvas fiber as the catalyst in non-aqueous media.The effects of reaction conditions,such as solvent,acyl donor,temperature and substrate ratio on the catalytic activity（EA3） of 3 and the enantioselective ratio（E4） of 4 were investigated.Under the optimum conditions,3 showed a satisfying catalytic performance（EA3=0.53 μmol·min-1·mg-1,E4=15.4）,and 3 was recycled eight times with slight decrease in the catalytic performance.
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