中文摘要：

A novel method for analysis of three active components curcumin,demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L.was developed by HPLC coupled with electrochemical detection.Three curcuminoids were well separated on a C18 column and detected with high sensitivity.A mobile phase containing acetonilrile and 10 mM Na2HPO4-H3PO4（pH 5.0）（50:50.v/v） was used.Good linearity was obtained in the range of 0.208-41.6,0.197-39.4,and 0.227-114 μM for curcumin.demethoxycurcumin and bisdemethoxycurcumin respectively.The limit of detection reached up to 10-8 M.which was lower than that by UV detection.The relative standard deviations（RSDs） ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision,respectively.The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.

英文摘要：

A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4-H3PO4 （pH 5.0） （50：50, v/v） was used. Good linearity was obtained in the range of 0.208-41.6, 0.197-39.4, and 0.227-114μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10 ？ 8 M, which was lower than that by UV detection. The relative standard deviations （RSDs） ranged from 1.06%to 1.88%for intra-day precision and from 4.30%to 5.79%for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3%to 111%were obtained.