中文摘要：

目的：观察不同浓度外源性甲胎蛋白（AFP）对AFPsiRNA-HepG2细胞系（AFP表达缺失HepG2细胞系）的增殖与凋亡的影响。方法：体外培养AFPsiRNA-HepG2细胞,将细胞分为对照组,100μg/ml、10μg/ml、1μg/ml、0.1μg/ml、0.01μg/ml AFP 6组,MTT法检测细胞增殖;Annexin V-APC/7-AAD染色、流式细胞术检测细胞凋亡百分比。结果：MTT结果提示,与阴性对照组比较,100μg/ml AFP组显著抑制细胞增殖（P〈0.01）,低于100μg/mlAFP组促进细胞增殖（P〈0.05）;流式细胞术结果提示,与阴性对照组比较,100μg/ml AFP组显著诱导细胞凋亡（P〈0.01）,低于100μg/ml AFP干预组对细胞凋亡无影响（P〉0.05）。结论：低于100μg/ml AFP可促进细胞增殖,高于100μg/ml AFP可显著抑制细胞增殖,并诱导其发生凋亡。

英文摘要：

Objective： To determine the effects of exogenous AFP on the proliferation and apoptosis in AFP siRNA-HepG2 cells.Methods： AFP siRNA-HepG2 cells were cultured and stimulated by AFP with the concentrations of 100μg / ml,10μg / ml,1μg / ml,0.1μg / ml and 0.01μg / ml respectively.The proliferation was evaluated by MTT assay.Apoptosis was stained with Annexin V-FITC / PI Kit and determined by flow cytometry.Results： MTT assay showed that 100μg / ml of AFP markedly suppressed the proliferation of AFP siRNA-HepG2,while 10μg / ml,1μg / ml,0.1μg / ml and 0.01μg / ml of AFP promoted the proliferation of cells.Flow cytometry assay suggested that 100μg / ml of AFP induced the apoptosis of AFP siRNA-HepG2 cells,while the other concentrations of AFP had no effect on cells.Conclusion： Low concentration of AFP can promote the proliferation of AFP siRNAHepG2 cells,while high concentration of AFP suppresses the proliferation and induces the apoptosis of AFP siRNA-HepG2 cells.