中文摘要：

[目的]探讨正肝方对黄曲霉毒素B1（AFB1）诱发的大鼠癌前病变肝组织γ-谷氨酰转移酶（GGT）、谷胱甘肽S-转移酶-π（GST-P）阳性灶的影响。[方法]Wistar大鼠100只随机分为4组：模型组30只、正肝方小剂量预防（小剂量）组30只、正肝方大剂量预防（大剂量）组30只、正常大鼠对照（正常）组10只。除正常组外，先后用AFB1和2-乙酰氨基芴（2-AAF）处理各组大鼠造模。大、小剂量组在造模期间，将正肝方水剂（0．6g／ml，0．3g／ml）按10ml／kg分别灌胃；模型组用无菌蒸馏水10ml／kg灌胃。8周后处死大鼠，肝组织取材。组织化学法做肝组织GGT染色、免疫组化法做肝组织GST-P染色，电脑图像分析系统测量GGT和GST-P阳性灶的数量、大小。[结果]大剂量组大鼠肝组织GGT阳性灶为（10．91±4．25）个／cm^2，灶总面积为（2．94±1．52）mm^2／cm^2；GST-P阳性灶为（24．75±10．99）个／cm^2，灶总面积为（4．80±2．75）mm^2／cm2，均显著低于模型组（P〈0．01）。[结论]正肝方能减少AFB1诱发的大鼠癌前病变肝组织GGT和GST-P阳性灶的数量和大小，对AFB1诱发的肝癌前病变具有一定的预防作用。

英文摘要：

[Objective]To observe the effects of Zhengganfang（ZGF）on AFB-1-induced γ-glutamyltranspeptidase-positive hyperplastic liver cell foci （positive GGT foci）and glutathione Stransferase pi-positive hyperplastic liver cell foci（positive GST-P foci）in rat hepatic precancerous lesion tissue. [Methods]lOne hundred rats were divided into four groups：control group of model, group of ZGF low dosage, group of ZGF high dosage and control group of normal rats. Except the control group of normal rats,all rats in each group were treated with AFB-1 and 2-AAF successively for making model. During the period of making model, the rats in group of ZGF low dosage and group of ZGF high dosage were fed with ZGF at different dosage. After eight weeks,the autopsied liver tissues from each rat were checked for the number and size of positive GGT foci and positive GST-P loci. [Results]The number（per cm^2 ）and the size（mm^2/cm^2）index of positive GGT foci of rat liver in group of ZGF high dosage were 10.91±4. 25 and 2. 94±1.52. The number（per cm^2 ） and the size（mm^2/cm^2 ） index of positive GST-P loci of rat liver in group of ZGF high dosage were 24. 75±10. 99 and 4.80±2. 75. Both of them were lower than that in control group of model （P〈0. 01）. [Conclusion]ZGF can inhibit the number and size of AFB-1-induced positive GGT loci and positive GST-P loci,and ZGF has the function of preventing AFB-l-indueed precancerous lesion of liver.