中文摘要：

Cryotop冷冻技术是口前卯母绌胞玻璃化保存较为有效的方法，它以操作简单、获得的冷冻速率较高而得到，“泛应用。近年来研究者们采用数值计算的方式对其传热性能进行研究，但计算中涉及的模型冷冻过程中的对流换热系数却没有有依据的取值范围。文章以_二种载板厚度的Cryotop模型为研究对象，采片超快速测温装置对水滴冷冻过程进行温度测量，同时刚模拟计算的方法得到不同对流换热系数下水滴冷冻过程的冷却速率，将两者进行比较，找出与实际测温所得结果相吻合的模拟实验中所需要的对流换热系数取值范围。结果表明：一种小同载板厚度的Cryotop模型在模拟计算中对流换热系数的取定范围为6000～8000W／（m^2·K）。

英文摘要：

The Cryotop is an efficient vitrification method for cryopreservation of oocytes. It has been widely used owing to its simple operation and high freezing rates. Recently, its heat transfer performance is frequently studied by the way ot numerical slmmauon. However, the range of heat transfer coefficient in the simulation is uncertain. Three kinds of Cryotop models with different board thickness were studied. The cooling rates of water droplets in freezing process of Cryotop were measured by ultra-fast measurement and calculated by numerical simulation. Comparing the results obtained by two methods, the range of the heat transfer coefficient which was needed in the numerical simulation of Cryotop was found. The result showed that the range of the heat transfer coefficient is 6000-8000 W/（m^2·K）.