中文摘要：

卵母细胞的冷冻保存已经成为女性生殖力保存的一个重要方式，在人类辅助生殖领域中起着关键的作用。近几年，卵母细胞冷冻保存技术的研究有了很大的突破，在诸多方面都提出了一些新技术与新方法，主要包括冷冻方法、保护剂种类、保护剂添加与去除的方式以及卵母细胞质量的评价等，其中微流体装置不仅用于保护剂的连续添加和去除，还能用于细胞的玻璃化冷冻保存，同时细胞质量评价技术的优劣直接会影响胎儿的发育，甚至成人的健康。因此，微流体技术与科学的质量评价方法可能是未来卵母细胞冷冻保存研究的重要方向。

英文摘要：

Oocyte cryopreservation has become an essential meams in fertility preservation for women, and plays a key role in the field of human assisted reproduction. In recent years, the research of oocyte cryopreservation technology has made a significant breakthrough, and put forward some new technologies and new methods in many ways, mainly including freezing method, types of cryoprotectants, the way of the cryopro- tective agent （CPA） loading and unloading, and oocyte quality assessment and so on. The microfluidic device is not only used for CPA continuous loading and unloading, also used for cell vitrification preservation, and the advan- tages and disadvantages of cell quality evaluation technology can directly affect the fetal developmental, even the adult health. So microfluidic technology and scientific quality evaluation method may be an important direction for oocyte cryopreservation in the future.