中文摘要：

研究了酿酒酵母和嗜鞣管囊酵母对油菜秸秆稀酸水解抑制物糠醛、5-羟甲基糠醛和乙酰丙酸的耐受性。酿酒酵母能够耐受分别含有2 g/L糠醛、2 g/L 5-羟甲基糠醛和8 g/L乙酰丙酸的葡萄糖发酵液;分别含有2 g/L糠醛、2 g/L 5-羟甲基糠醛和8 g/L乙酰丙酸的木糖发酵液严重抑制嗜鞣管囊酵母发酵木糖产乙醇。在含有2 g/L糠醛的葡萄糖发酵液中,酿酒酵母和嗜鞣管囊酵母的乙醇质量浓度分别为对照的85.05%和46.70%,而在以木糖为底物的发酵液中,嗜鞣管囊酵母的乙醇质量浓度为对照的12.40%。油菜秸秆水解液能够有效缓解2 g/L糠醛对酿酒酵母和嗜鞣管囊酵母产乙醇的抑制,乙醇质量浓度分别为对照的98.40%和91.00%。研究结果表明,酿酒酵母对抑制物糠醛、5-羟甲基糠醛和乙酰丙酸的耐受性高于嗜鞣管囊酵母。

英文摘要：

The tolerance of Saccharomyces cerevisiae and Pachysolen tannophilus to inhibitors including furfural,5-hydroxymethyl furfural and levulinic acid in diluted acid hydrolysis from rape straw was investigated.S.cerevisiae was capable of tolerating 2 g/L furfural,2 g/L 5-hydroxymethyl furfural and 8 g/L levulinic acid in glucose broth.However,ethanol production by P.tannophilus was strongly inhibited in xylose broth added with 2 g/L furfural,2 g/L 5-hydroxymethyl furfural and 8 g/L levulinic acid,respectively.In glucose broth containing 2 g/L furfural,the ethanol yields of S.cerevisiae and P.tannophilus were respectively 85.05% and 46.70% compared with those of the control,while in xylose broth added with 2 g/L furfural,the ethanol yield of P.tannophilus was 12.40% compared with that of the control.Enzymatic hydrolysis of rape straw could effectively relieve the inhibition of 2 g/L furfural on both the S.cerevisiae and P.tannophilus,whose ethanol yields were respectively 98.40% and 91.00% compared with those of the control.The results showed that the tolerance capacity of S.cerevisiae on fermentation inhibitors including furfural,5-hydroxymethyl furfural and levulinic acid was stronger than that of P.tannophilus.