中文摘要：

为加快贵州长顺绿壳蛋鸡选育与开发利用,采用PCR-SSCP和DNA测序技术对124只长顺绿壳蛋鸡的雌激素受体1（estrogen receptor,ESR1）基因进行多态性分析,并与其蛋品质性状进行关联性研究。结果表明,长顺绿壳蛋鸡ESR1基因中出现AA、AC和CC 3种基因型,其由于A（-999）C 1个SNPs所导致,经χ2适合性检验,基因型分布偏离了Hardy-Weinberg平衡（P〈0.05）。基因型与蛋品质性状关联性分析结果显示,3种基因型个体间的蛋重、蛋形指数、蛋黄比率、蛋白比率、蛋壳重差异不显著（P〉0.05）,而AA与AC基因型个体的蛋壳厚度差异显著（P〈0.05）,在不影响其它性状的情况下,选择AA基因型个体可得到良好的蛋壳厚度,从而增强蛋品质。

英文摘要：

In order to quicken breeding and exploitation of Guizhou Changshun Blue-shell chickens,PCR-SSCP and DNA sequence technology were used to analyze polymorphisms and association of estrogen receptor 1（ESR1） gene polymorphisms with egg quality from 124 Changshun Blue-shell chickens.The results showed that there were three kinds of genotypes（AA,AC and CC） in ESR1 gene resulted from A（-999）C,the distribution of genotypes deviated from Hardy-Weinberg equilibrium（P〈0.05） by Chi square test,the difference among egg weight,egg shape index,egg yolk and albumen ratio,eggshell weight was not significant（P〈0.05）,whereas the difference of eggshell thickness between AA and AC genetypes was significant individuals of AA genotype could get better eggshell thickness,which might strengthen egg quality.