将质量浓度为400ug/mL的EGCG及EGCG-3Me添加到全酸蚀粘接剂single Bond2(SB2)中,制备改性粘接NE-SB2及E3-SB2,SB2为对照组。激光共聚焦显微镜和分光光度法检测改性粘接剂抗粪肠球菌的性能;微拉曼光谱仪检测粘接剂双键转化率;制各纤维桩粘接试件,用于即刻和老化后的微推出实验。结果表明,改性粘接剂可以抑制粪肠球菌生物膜形成,且EGCG-3Me作用更显著;改性粘接剂与SB2的双键转化率和即刻微推出粘接强度差异无显著性(P〉0.05);老化后改性粘接剂的微推出粘按强度显著高于SB2(P〈0.05)。
Epigallocatechin-3-gallate (EGCG) and epigallocatechin-3-O-(3-O-methyl)-gallate ( EGCG-3Me ) was incorporated into the total-etch adhesive of Single Bond 2 separately to obtain two modified adhesives E-SB2 and E3- SB2 with the concentration of 400 μg/mL of EGCG and EGCG-3Me respectively, The confocal laser scanning microscopy (CLSM) and the ultraviolet spectrophotometry were used to evaluate the anti-bacterial effect of modified adhesives. The micro-Raman spectrum was used to test the degree of double bond conversion (DC) of adhesives. The push-out bond strength test was conducted to test the immediate bonding strength and the bonding strength after thermocycling. The results demonstrated that E-SB2 and E3-SB2 both showed inhibiting effect to the growth and activity of E.faecalis, while E3-SB2 performed stronger inhibiting effect. The DC and immediate push-out bonding strength of SB2 were not decreased with the incorporation of EGCG or EGCG-3Me (P〉0.05). E-SB2 and E3-SB2 showed significantly higher push-out bonding strengths than that of SB2 (P 〈 0.05).