中文摘要：

细胞的区别能被细胞外的环境影响，特别地细胞外的底层。底层的 nanotopography 可以在 vivo 涉及细胞的区别的机制。细胞器是在各种各样的细胞的函数的主要播放器；然而，细胞器上的 nanotopography 的影响还没被阐明了。在现在的学习， micropit-nanotube 地形学(MNT ) 在钛表面上被制作，并且细胞器特定荧光灯探针被用来检测 MG63 房间的细胞内部的细胞器组织。在细胞器之间的通讯，由细胞器特定的 GTPase 表示识别了，被量的聚合酶链反应并且西方的弄污评估。传播电子显微镜学被执行评估细胞器结构。在在 MNT 和扁平的表面之间的细胞器分发或数字没有重要差别。然而， MNT 上的细胞器特定的 GTPases 是戏剧性地 downregulated。另外，明显的 endoplasmic 蜂窝胃腔膨胀在 MNT 表面上被观察，并且展开的蛋白质反应(UPR ) 也被开始。关于在细胞器 trafficking， UPR，和 osteogenic 区别之中的关系，我们的调查结果可以提供重要卓见进 nanotopography 导致的信号 transduction。

英文摘要：

Cellular differentiation can be affected by the extracellular environment, particularly extracellular substrates. The nanotopography of the substrate may be involved in the mechanisms of cellular differentiation in vivo. Organelles are major players in various cellular functions; however, the influence of nano- topography on organelles has not yet been elucidated. In the present study, a micropit-nanotube topography （MNT） was fabricated on the titanium surface, and organelle-specific fluorescent probes were used to detect the intracellular organelle organization of MG63 cells. Communication between organelles, identified by organelle-specific GTPase expression, was evaluated by quantitative polymerase chain reaction and western blotting. Transmission electron microscopy was performed to evaluate the organelle structure. There were no significant differences in organelle distribution or number between the MNT and flat surface. However, organelle-specific GTPases on the MNT were dramatically downregulated. In addition, obvious endoplasmic reticulum lumen dilation was observed on the MNT surface, and the unfolded protein response （UPR） was also initiated. Regarding the relationships among organelle trafficking, UPR, and osteogenic differentiation, our findings may provide important insights into the signal transduction induced by nanotopography.