中文摘要：

目的克隆并表达HDAg基因，确定HDAg蛋白在细胞内定位。方法常规分子克隆、错配PCR、Westernblot、RT-PCR刀免疫细胞化学等。结果通过RT-PCR及错配PCR方法从1例HBV／HDV重叠感染患者血清中成功克隆出HDAg基因，生物信，∈学分析证实其来源于我国流行的基因I型河南株。将HDAg基因克隆于真核载体转染肝细胞，证实两种不同形式的HDAg主要矗位在细胞核。结论HDAg定位于细胞核可能是其发挥相应生物学作用的基础。

英文摘要：

Objective To identify the cellular localization of HDAg protein after the cloning and expression of HDAg gene. Methods Routine molecular cloning, mismatch amplification mutation assay, Western blot, RT-PCR and immu nofluorescenee assay were adopted. Results In this study, HDAg gene was cloned from a patient＇ s serum co-infected with HBV/HDV. The analysis of bioinformaties supported the evidence of genotype I from Henan strain. The entire cod ing sequence of HDAg was cloned into an eukaryotic vector. The expression vector was transfected into human liver HepG2 cells. Expression products of both L-HDAg and S-HDAg were observed to loealizate in the nuclei by immunofluo rescence assay. Conclusion HDAg may play an important role through its cell localization in the nucleus.