中文摘要：

目的探讨Aβ1~42及二氮嗪预干预对神经细胞Na+-K+-ATP酶β亚基蛋白表达的影响。方法原代培养大鼠皮层海马神经细胞,随机分为空白对照组、Aβ1~42组(2μmol/L)、二氮嗪(50μmol/L)预处理1 h后Aβ1~42组、单独二氮嗪预处理组,各组又分为24、72 h两个时间点,采用免疫荧光及免疫印迹法检测干预后不同培养时间细胞Na+-K+-ATP酶β亚基蛋白表达水平的变化。结果免疫荧光显示:Aβ1~42作用细胞72 h降低了Na+-K+-ATP酶β亚基荧光强度;而经二氮嗪预处理后Aβ1~42作用72 h,与单独Aβ1~42组相比较荧光强度有所增强。Western印迹显示:二氮嗪预处理神经细胞1 h协同Aβ1~42作用24 h后及单独二氮嗪组Na+-K+-ATP酶β亚基的蛋白表达明显低于对照组及单独Aβ1~42组(P<0.01)。Aβ1~42作用神经细胞72 h后,Na+-K+-ATP酶β亚基蛋白表达较对照组显著降低(P<0.05);二氮嗪预处理1h协同Aβ1~42共同作用于神经细胞72 h后,与单独Aβ1~42组相比较,增加了Na+-K+-ATP酶β亚基蛋白表达量(P<0.05)。结论 Aβ1~42作用72 h,Na+-K+-ATP酶β亚基蛋白表达量降低,二氮嗪预处理1 h协同Aβ1~42共同作用于神经细胞72 h,Na+-K+-ATP酶β亚基蛋白表达量升高。

英文摘要：

Objective To investigate the effects of diazoxide and Aβ1~42 on Na+-K+-ATPase β-subunit protein expression in neu-rons.Methods Primary neurons were cultured and evaluated by immunoctochemistry, then neurons were randomly divided into control, Aβ1~42 ,diazoxide+Aβ1~42 ,diazoxide groups.After treatment for 24 h or 72 h,Na+-K+-ATPase β-subunit was determined by immunofluores-cence and immunoblotting.Results Being exposed to Aβ1~42 for 72 h, the expression of Na+-K+-ATPase β-subunit was significantly re-duced.Compared with that of Aβ1~42 group, the Na+-K+-ATPase β-subunit expression was significantly up-regulated in diazoxide+Aβ1~42 group for 72 h.Compared with that of control group and Aβ1~42 group,the protein expression level of Na+-K+-ATPase β-subunit in both di-azoxide+Aβ1~42 group and diazoxide group were significantly reduced for 24 h(P<0.01).Being exposed to Aβ1~42 for 72 h,the protein ex-pression of Na+-K+-ATPaseβ-subunit was significantly decreased(P<0.05).Compared with that of Aβ1~42 group,the level of Na+-K+-AT-Paseβ-subunit was significantly increased in diazoxide+Aβ1~42 group for 72 h(P<0.05).Conclusions Aβ1-42 could reduced the protein ex-pression level of Na+-K+-ATPaseβ-subunit in cultured primary neurons 72 h,and the Na+-K+-ATPaseβ-subunit is significantly increased in diazoxide+Aβ1~42 group for 72 h.