中文摘要：

目的观察高碘对哺乳期甲状腺疾病患者外周血促甲状腺激素受体（TSrtR）和蛋白激酶A（PKA）及钠碘同向转运体（NIS）mRNA表达的影响。方法在山西省适碘和高碘地区，抽取99名哺乳妇女作为观察对象。并按照是否患有甲状腺疾病分为病例组和对照组（高碘地区病例组21名、高碘地区对照组19名、适碘地区病例组30名、适碘地区对照组29名）。采集所有观察对象外周血，采用实时荧光定量PCR法检测TSHR、PKA、NISmRNA表达。结果高碘地区病例组[中位数（M）：0．099、0．994]和对照组（M：0．240、0．738）分别与适碘地区病例组（肘：3．087、1．127）和对照组（M：1．823、0．842）比较，鸭HRmRNA表达明显降低（z=-5．034、-4．010，P均〈0．01），PKAmRNA表达有降低趋势，但差异无统计学意义（Z=2．895、-0．343，P均〉0．05）。高碘地区病例组NISmRNA表达（M：0．485）明显低于适碘地区病例组（M：2．680，z=-3．311，P〈0．01），而高碘地区对照组（M：0．470）与适碘地区对照组（肘：0．835）比较，差异无统计学意义（Z=-1．882，P〉0．05）。NIS、，I’sHRmRNA表达呈正相关[相关系数（r）=0．741，P〈0．01]；NIS、PKAmRNA表达也呈正相关（r=0．293，P〈0．01）；但TSHR、PKAmRNA表达未见统计学相关性（r=-0．081，P〉0．05）。结论哺乳期妇女可能通过促甲状腺激素（TSH）．TsHR．环磷酸腺苷（cAMP）．PKA信号通路调控机体的摄碘水平来达到保护自身及子代的目的。

英文摘要：

Objective To observe the effect of high iodine on mRNA expression of thyroid hormone receptor （TSHR）, protein kinase A （PKA） and sodium iodide symporter （NIS） in peripheral blood of patients with thyroid diseases during lacatation. Methods A total of 99 breast-feeding women were selected as observation objects in Shanxi Province＇s sufficient, iodine and high iodine areas, and they were divided into case group and control group according to whether suffer from thyroid disease. In high iodine areas, there were 21 patients and 19 healthy controls. In sufficient iodine areas, there were 30 patients and 29 healthy controls. Peripheral blood of all the observation objects was collected, and mRNA expression of TSHR, PKA and NIS was detected by real-time quantitative PCR. Results The case group [median （M）： 0.099, 0.994] and the control group （M： 0.240, 0.738） in the high iodine areas were respectively compared with the case group （M： 3.087, 1.127） and the control group （M： 1.823, 0.842） in the sufficient iodine areas. The TSHR mRNA expression was significantly decreased （Z = - 5.034, - 4.010, all P 〈 0.01）; the PKA mRNA expression had a downward trend, and the difference was not statistically significant （Z = 2.895, - 0.343, all P 〉 0.05）. The NIS mRNA expression of the case group in high iodine areas （M： 0.485） was obviously lower than that of the the case group in sufficient iodine regions （M： 2.680, Z =- 3.311,P 〈 0.01）. The control group in high iodine areas （M： 0.470） was compared with the control group in sufficient iodine areas （M： 0.835）, and the difference was not statistically significant （Z = - 1.882, P 〉 0.05）. The NIS and the TSHR mRNA were positively correlated [correlation coefficient （r） = 0.741, P 〈 0.01]; the NIS and the PKA mRNA was also positively correlated （r = 0.293, P 〈 0.01）; but the TSHR mRNA was not significantly correlated with the PKA mRNA （r = - 0.081, P 〉 0.05）. Conclusion Lactating women ma