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中文摘要：
目的：为了将抗树突状细胞（dendritic cells,DC）抗体应用于临床疾病治疗,计划克隆获得抗人DC单克隆抗体（McAb）的可变区序列,在此基础上进一步获得人源化的抗人DC抗体。方法：运用RT-PCR技术,从分泌抗人DC McAb的杂交瘤细胞株（H1E11）中,克隆出VH和VL基因片段,并对VH和VL进行了测序分析。所测得的重链和轻链在基因库中与相关的Ig的VH和VL基因序列进行经Blast同源性比较。结果：通过碱基和蛋白序列的比较确认为小鼠IgG的VH和VL基因。运用Dnasis软件对VH和VL基因进行阅读框分析和模拟翻译,并参照Kabat等人（1991）编写的Ig蛋白质编码序列图谱,根据其FRs和CDRs的序列特征进行Ig的家族分析,发现VH共有414 bp,编码138个氨基酸序列,属于小鼠IgG重链Ⅲ（A）家族;VL由396 bp编码,产生132氨基酸,属于轻链κV家族。结论：成功克隆抗人DC McAb重链和轻链的可变区。
英文摘要：
Objective： For applying the antibody to treat clinical disease, to get humanized antibody. Methods： Using RT-PCR technique to clone the genes of VH and VL from the hybridom cell strain H1E11, Then we assayed their sequences, and used blast tool to compare them in the GenBank. Results： We found that the VH and VL were similar to the VH sequence of IgG and VL sequence of K respectively. At the end we analyzed and identified them with Dnasis software or according to ＂Sequence of protein of immunological interest＂ which edited by Kabat EA and found that VH and VL belong to mouse IgG heave chain Ⅲ （A） family and light chain KV family respectively. Conclusion： We have cloned VH and VL gene from human anti- DC McAb successfully.
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