中文摘要：

目的：分析胃癌患者Th1和Th2两类细胞因子的基因表达与转录因子T-bet和GATA3表达的相关性,从细胞因子与转录因子角度考证胃癌患者Th1/Th2细胞分化趋势,探讨p53与T-bet的相关性,了解T-bet与肿瘤转移之间的关系。方法：利用荧光定量PCR技术检测55例胃癌患者及45例正常人外周血单个核细胞（PBMC）中T-bet、GATA3转录因子和IFN-γ、IL-4等细胞因子的水平,应用免疫组化法检测胃癌组织中p53的表达状况。结果：55例胃癌患者T-bet、GATA3、IFN-γ、IL-4的表达率依次为51%（28/55）、78%（43/55）、27%（15/55）、69%（38/55）。定量PCR结果显示,病人组的T-bet和IFN-γ明显低于正常对照组（P〈0.01）,而GATA3和IL-4则明显高于正常对照组（P〈0.01）。T-bet与IFN-γ在病人体内的mRNA表达存在正相关性（P〈0.01）,正常人则没有。GATA3与IL-4的表达在两组中均呈明显正相关（P〈0.01和P〈0.05）,且在p53阳性的患者中,T-bet的表达率较低,而GATA3的表达率较高。结论：胃癌患者有明显Th2漂移现象,且p53的表达与T-bet的表达呈负相关。

英文摘要：

Objective：To confirm differentiation of Th1/Th2 on the levels of transcription and cytokines production in patients with gastric carcinoma, and to reveal the relationship between the expression of T-bet/GATA3 and Thl/Th2 type cytokines in peripheral blood mononuclear cells （PBMC）. In addition, the relation of p53 and T-bet expression was discussed for understanding the role of T- bet in metastasis. Methods：The gene expression of Thl/Th2 type cytokines IFN-γ IL-4, transcription factors T-bet/GATA3 were determined by RT-PCR and realtime RT-PCR from 55 patients with gastric cancer and 45 normal person. The expression of p53 was analyzed by immunohistochemistry. Results： Predominant expression of Th2 type cytokines was detected in 55 gastric cancer patients. The expression rates of T-bet, GATA3 ,IFN-γ and IL-4 was 51% （28/55）, 78% （43/55） ,27% （15/55）and 69% （38/55）, respectively. The expression rates of T-bet and IFN-γ in patients were lower than control groups, but the results of GATA3 and IL-4 were opposite. The similar results were obtained by realtime PCR. There was a positive correlation between the expression of T-bet and IFN- γ in patient, but no relationship in normal person. The positive correlation between GATA3 and IL-4 was also found in patients and in normal person. The expression of p53 in patients was accompanied with lower expression rate of T-bet and higher expression of GATA3. Conclusions： Predominant expression of Th2 type cytokines may be related to lower expression of T-bet or higher expression of GATA3 in PBMC of gastric cancer patients. The negative correlation exists between p53 and T-bet.