中文摘要：

以Poly（I）-Poly（C）诱导鸡成纤维细胞Mx基因的表达，提取总RNA，RT—PCR扩增出全长Mx cDNA，扩增产物克隆入pMD19-T Simple载体中进行序列测定。结果表明：与GenBank公布的鸡MxcDNA序列相比，其同源性达99．9％，为进一步研究鸡Mx基因的抗病毒活性和作用机理奠定了基础。

英文摘要：

A full-length cDNA of chicken Mx gene was obtained using reverse transcription polymerase chain reaction （RT-PCR） amplification of total RNA extracted from chicken embryo fibroblast （CEF） which was induced with Poly（I）- Poly（C）. The RT-PCR product was subcloned into pMD19-T simple vector and the correctness of the sequences was confirmed by sequence analysis. Result of sequence analysis showed that its homology with Mx cDNA from GenBank was up to 99.9%. These data indicated that the full-length Mx cDNA was cloned correctly, which will play a great role in research of its antiviral function and mechanism.