中文摘要：

目的评价咪达唑仑对人离体精子能动性的影响。方法正常精液标本经percoll密度梯度法离心优选后沉淀以新鲜培养基重悬，密度调至（10—30）×106／ml，采用随机数字表法，将其随机分为3组（n=10）：对照组（c组）加入生理盐水正常培养；不同临床浓度咪达唑仑组（M1组和M：组）分别加入咪达唑仑使其终浓度为5、1ttg／ml；C组、M。组和M：组于加入咪达唑仑后10、30、60min时采用计算机辅助精液分析系统评价精子能动性，记录精子运动活力、曲线运动速度、直线运动速度、路径运动速度、头部侧摆幅度、鞭打频率、直线性、摆动性、前向性和移动角度。结果组内组间比较，精子能动性各参数差异均无统计学意义（P〉0．05）。结论临床浓度咪达唑仑对人离体精子能动性无明显影响。

英文摘要：

Objective To investigate the effect of midazolam on human sperm motility in vitro. Methods Sperm samples were obtained from normal adults and prepared with discontinuous percoll gradient centrifugation technique. The samples were randomly divided into 3 groups （ n = 10 each） ： control group and 2 midazolam groups. The samples were incubated with normal saline in control group and with midazolam with the final concen- trations of 5 or 1 μg/ml in 2 midazolam groups. The samples were incubated for 60 min in an airtight container at 37 ℃. Then human sperm motility was examined in vitro at 37 23 and analyzed by the computer-assisted sperm analysis at 10, 30 and 60 min exposure to midazolam, including sperm motility （a ＋ b）%, curvilinear velocity, straight line velocity, average path velocity, amplitude of lateral head displacement, beat-cross frequency, lineari- ty, wobble, straightness, and mean angular displacement. Results There was no significant difference in the pa- rameters of human sperm motility within each group and between groups （ P 〉 0.05）. Conclusion Midazolam has no significant effect on human sperm motility in vitro.