中文摘要：

这研究在有教养的发展中的老鼠在 NMDA 受体电流(INMDA ) 和细胞色素 C 的表示的振幅上检验了 isoflurane 的临床上相关的集中的效果海马趾的神经原。海马从新生的 Sprague-Dawley 老鼠被把。海马趾的神经原主要是有教养的 5 天了然后与 isoflurane 的不同集中对待[(0.25， 0.5， 0.75， 1 最小的牙槽的集中(MAC ))] 。INMDA 的山峰借助于整个房间补丁被记录夹钳技术。细胞色素 C 水平被西方的弄污和量的即时 PCR 检测。我们的结果证明 isoflurane (0.25， 0.5， 0.75 和 1 MAC ) 加强了 INMDA 由的振幅(116 吗？

英文摘要：

This study examined the effects of clinically relevant concentrations of isoflurane on the amplitude of NMDA receptor current （INMDA） and the expression of cytochrome C in cultured developing rat hippocampal neurons. The hippocampi were dissected from newborn Sprague-Dawley rats. Hippocampal neurons were primarily cultured for 5 days and then treated with different concentrations of isoflurane [（0.25, 0.5, 0.75, 1 minimum alveolar concentration （MAC））]. The peak of INMDA was re- corded by means of the whole cell patch clamp technique. The cytochrome C level was detected by Western blotting and quantitative real-time PCR. Our results showed that isoflurane （0.25, 0.5, 0.75 and 1 MAC） potentiated the amplitude of INMDA by （116±8.8）%, （122±11.7）%, （135±14.3）% and （132~14.6）%, respectively, and isoflurane increased the mRNA expression of cytochrome C in a concentration-dependent manner. The cytochrome C mRNA expression reached a maximum after 0.5 MAC isoflurane stimulation for 6 h （P〈0.05）. It was concluded that isoflurane enhances the expression of cytochrome C in cultured rat hippocampal neurons, which may be mediated by facilitation of NMDA receptor.