中文摘要：

比较了城市污水中磺胺二甲基嘧啶（SM2）的两种检测方法,即酶联免疫（ELISA）法和SPE-HPLC法。HPLC法以AgilentZORBAX Exlipse XDB-C18色谱柱为分析柱,流动相乙腈与超纯水的比例为2：8（V：V）,270 nm处检测SM2含量。结果表明,ELISA法的平均回收率为90.4%,相对标准偏差（RSD）为2.6%,方法的检测限为4 ng/mL。HPLC的平均回收率为93.7%,方法检测限为5 ng/mL。用上述两种方法测定松江污水处理厂进出水中SM2的浓度,结果用两种方法在进水中都检测到了SM2,而在出水中未检出,且水中的杂质使ELISA测定结果偏大。当水样经SPE前处理,ELISA法与HPLC法测定结果无显著性差异。

英文摘要：

Enzyme-linked immunosorbent assay（ELISA）was compared with high performance liquid chromatography（HPLC）for trace determination of sulfamethazine in municipal sewage.For HPLC,Agilent ZORBAX Exlipse XDB-C18 column was applied for separation of the investigated sulfamethazine.Acetonitrile and ultrapure water were used as the mobile phase,and the wavelength of determination was 270 nm.Results showed that average recovery of ELISA was 90.47%,RSD was 2.6% and limit of detection was 4 ng/mL.HPLC average recovery of SM2 was 93.7% with the limit of detection 5 ng/mL.The two methods were applied to detect the sulfamethazine in municipal sewage of Songjiang WWTP.Results indicated that the two methods all detected SM2 in the inflow while SM2 was not detected in the outflow.Result also indicated that the impurities in the water interfered ELISA determination,which overestimated the data.However,after pretreatment of SPE,there was no significant difference between the two methods according to the determination results.