中文摘要：

使用西方蜜蜂雄蜂头部5’LongSAGE文库中的肌球蛋白调节性轻链基因MLC-2的标签序列,在蜜蜂全基因组序列上定位了该基因的转录起始位点（TSS）,并进而预测了其启动子的结构。结果显示：蜜蜂MLC-2基因存在17个TSS,其中16个定位于MLC-2基因开放阅读框上游100bp的范围内。MLC-2基因的各TSS的使用效率不同,其中有3个优势TSS,由之起始的转录本分别占该基因总转录本数量的9.76%、54.47%和11.38%。从碱基组成来看,蜜蜂MLC-2基因TSS的第1个碱基为A、G、T、C的概率分别为58.8%、29.4%、0和11.8%。MLC-2基因的启动子结构预测结果表明,在TSS上游的300bp区域内有3个核心启动子元件。研究结果对研究蜜蜂MLC-2基因的表达调控与确定其全长cDNA序列具有重要意义。

英文摘要：

The transcription initiation start site（TSS） of a predicted honeybee MLC-2 gene（myosin regulatory light chain 2）was localized on the genome sequence of Apis mellifera with the head of drone＇s 5＇Long-SAGE tags mapping to genome sequences in this study,and the structure of promoter was predicted.The results showed that there were 17 TSSs that were assigned to MLC-2 gene model and 16 tags that mapped in a 100 bp-region upstream of reference start codon.Interestingly,the authors found that those TSSs displayed different initiation efficiency and the MLC-2 mRNA transcripts were started from 3 dominant TSSs,which initiated 9.76%,54.47% and 11.38% respectively of the total transcripts of MLC-2 gene in adult drone head.When base composition of the first base of the 17 alternative TSSs was considered,the authors found that A,G,T and C accounted for 58.8%,29.4%,0 and 11.8%,respectively.Then,the promoter structure of MLC-2 gene was computational analyzed and 3 core promoter elements were predicted in DNA region of 300 bp upstream of TSSs.The precise identification of TSSs was fundamental for the establishment of full-length cDNA sequence and expression regulation of honeybee MLC-2 gene.