中文摘要：

目的通过观察少突胶质细胞系基因一髓鞘碱性蛋白（Golli-MBP）在口腔扁平苔藓（OLP）患者外周血单个核细胞中的表达，探索OLP的发病机制。方法采用密度梯度离心法分离20N充血糜烂型、16例光滑型OLP患者，以及19例对照组的外周血单个核细胞，分别用逆转录一聚合酶链反应（RT—PCR）法和Westernblot法检N3组研究对象外周血单个核细胞中Golli—MBPmRNA和蛋白的表达。结果充血糜烂型和光滑型OLP患者中Golli—MBPmRNA和蛋白的表达水平均高于对照组，且差异有统计学意义（P〈0．01）；但充血糜烂型和光滑型OLP患者组间表达的差异均无统计学意义（P〉0．05）。结论Golli—MBP在OLP的发病过程中起着重要作用。

英文摘要：

Objective The aim of this study was to determine the expression of genes of the oligodendrocyte lineagemyelin basic protein（Golli-MBP） in peripheral blood mononuclear cell （PBMC） in oral lichen planus（OLP） and to further understand the pathogenesis of OLP. Methods PBMC was obtained by density gradient centrifugation, and the expression of Golli-MBP in PBMC was investigated in erythernatous/erosive OLP （20 cases）, reticular OLP （16 cases） and normal controls（19 cases） using reverse transcription-polymerase chain reaction（RT-PCR） and Western blot methods. Results RT-PCR results showed that Golli-MBP mRNA was overexpressed in erythematous/erosive and reticular OLP as compared with normal control group（P〈0.01）. Western blot assay indicated that erythematous/erosive and reticular OLP patients had a higher expression level of Golli-MBP protein in PBMC than normal controls （/9〈0.01）. However, there were no significant differences between erythematous/erosive and reticular groups in Golli-MBP mRNA and protein expression （P〉0.05）. Conclusion The data accumulated here strongly indicate that Golli-MBP was involved in the pathogenesis of OLP.