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不同居群野生狗牙根材料的SSR分析
  • ISSN号:1007-0435
  • 期刊名称:草地学报
  • 时间:2013.5
  • 页码:598-606
  • 分类:S661.2[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]新疆草地资源与生态重点实验室新疆农业大学草业与环境科学学院,新疆乌鲁木齐830052
  • 相关基金:国家自然基金项目(31160483); 新疆科技厅高技术项目(20111112); 新疆农业大学国家重点学科项目(XJCYB-2011-05); 新疆农业大学校前期课题(XJAU201028)资助
  • 相关项目:新疆狗牙根抗寒分子标记的开发及抗寒基因克隆研究
中文摘要:

为评价新疆狗牙根(Cynodon dactylon)资源的遗传多样性水平,以采自新疆不同地域的狗牙根与部分华南地区及国外的狗牙根为材料,用SSR标记开展遗传多样性研究。结果表明:20对引物共产生142个等位变异,平均每个位点7个,其中有124个等位变异显示多态性,多态位点百分率为87.86%,位点Nei′s基因多样性(H)指数平均为0.4347;根据遗传一致性及遗传分化结果,106份狗牙根材料的遗传相似性系数值在0.43~1.0之间,平均0.715,居群间遗传分化系数为0.4119,表明有41.19%的变异存在于居群间,58.81%的变异存在于居群内,居群内的遗传分化大于居群间的遗传分化;根据UPGMA法聚类表明,106份供试材料相似系数为0.39时可聚为3类,材料间的遗传差异与其采集地的关系不很明显,但来自同一地区的材料较多地聚在一起,遗传背景较近,整体来看聚类结果与地理来源有一定的相关性。

英文摘要:

In order to evaluate the diversity level of Xinjiang Bermudagrass,the genetic diversities of Bermudagrass from Xinjiang,Huanan and abroad were investigated using SSR marker.Results indicated that 142 allelic variations were amplified by 20 primers,of which 124 were polymorphism and each locus had 7 allelic variations.The percentage of polymorphic loci was 87.86%,the average of Nei′s(H) was 0.4347.The genetic similarities(GS) of 106 materials ranged from 0.43 to 1.0,the average was 0.715.The genetic differentiation coefficient was 41.19% among the population and 58.81% inside the population.Data illustrated genetic variation within populations was larger than among populations according to genetic identity and differentiation.The general clustering consequences by UPGMA method indicated that 106 materials were classified into three groups when the similarity coefficient was 0.39.The genetic variance of Bermuda grass was not obviously related to the origin,but the materials from the same region were more classified into the same group.These clustering results were correlated with geographic origin in some extent.

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期刊信息
  • 《草地学报》
  • 中国科技核心期刊
  • 主管单位:中国科学技术协会
  • 主办单位:中国草原学会
  • 主编:王堃
  • 地址:北京市海淀区圆明园西路2号中国农业大学动科楼152室
  • 邮编:100193
  • 邮箱:cdxb@cau.edu.cn
  • 电话:010-62733894 62732799
  • 国际标准刊号:ISSN:1007-0435
  • 国内统一刊号:ISSN:11-3362/S
  • 邮发代号:80-135
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:13756