中文摘要：

目的：优选大黄煮散的粉碎粒度;通过与传统饮片的对比研究,确定大黄煮散的煎煮工艺。方法：制备3种规格的大黄煮散,采用HPLC法检测煮散水煎液中芦荟大黄素、大黄酸、大黄素、大黄酚及大黄素甲醚的含量,以化合物含量和干膏率为指标筛选大黄煮散粉碎粒度;采用单因素试验,考察加水量、煎煮时间对大黄煮散与传统饮片煎煮的影响。结果：首先,确定大黄煮散粒度为小于2 mm大于0.18 mm的颗粒在总颗粒中所占比例不得小于80%;其次,加水量14～20倍之间,大黄煮散总蒽醌含量与干膏率均无显著差异;煎煮时间对大黄煮散总蒽醌和干膏率无显著影响,但当煎煮时间超过20 min时,结合蒽醌的含量下降显著。煎煮时间对饮片影响较大,加10倍量水,煎煮1次,煎煮时间5～50 min,煮散化合物含量和干膏率分别是饮片的3.23～9.48倍、1.61～3.44倍,且随着煎煮时间的延长,差距呈缩小趋势。结论：筛选的大黄煮散制备工艺简单,适合工业生产;从药学角度来说,大黄饮片制成煮散,能节省一半以上的药材。

英文摘要：

Objective： To optimize the particle size of rhubarb boiling powder,to determine decoction process of rhubarb boiling powder through the comparative study with traditional herbal pieces. Methods： Three kinds of particle size of rhubarb boiling powder were prepared. The content of aloe emodin,rhein,emodin,chrysophanol and emodin monomethyl ether were detected by HPLC,and to determine particle size of rhubarb boiling powder according to the content of total chemical ingredients and dry extract rate in water decoction. The effect of the amount of water and decocting time on decoction of boiling powder and traditional herbal pieces of rhubarb were systematically investigated by using single factor test. Results： Firstly,the content of 2 mm to 0. 18 mm particles in rhubarb boiling powder can not be less than 80%. Total anthraquinone content and dry extract rate had no significant difference in the decoction of boiling powder in the amount of water between 14 ～ 20 times; decocting time had no significant effect on total anthraquinone and dry extract rate; the content of combined anthraquinone decreased significantly,while the decocting time was more than 20 min. The decocting time had great influence on the herbal pieces,with ten times of water,and decocting 5 ～ 50 min each time,the compound content and dry extract rate of boiling powder were 3. 23 ～ 9. 48 times and 1. 61 ～ 3. 44 times than that of traditional herbal pieces,and with the extension of the decocting time,the gap was narrowing. Conclusion： Preparation process of rhubarb boiling powder is simple and suitable for industrial production,boiling powder can save more than half of the amount of traditional herbal pieces with ten times of water,decocting one times,decoction time of 10 ～ 15 min.