中文摘要：

目的考察全氟辛烷磺酸（PFOS）暴露致大鼠肝损伤的作用及机制。方法48只Wistar大鼠随机分为对照组、低剂量组和高剂量组，分别给予含0、4、8mg／kgPFOS饲料染毒3个月。高效液相／质谱法检测大鼠血清和肝脏中PFOS含量；ELISA法测定各组丙氨酸转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、层粘连蛋白（LN）和羟脯氨酸（Hyp）含量变化。紫外分光光度法测定肝组织匀浆中单胺氧化酶（MAO）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、琥珀酸脱氢酶（SDH）活性变化。结果PFOS暴露致大鼠血清及肝脏中PFOS浓度显著增加，肝脏与血清中PFOS比值分别为（4．22±0．99）和（3．33±0．75）（P〈O．05）。PFOS暴露后大鼠肝脏重量和脏器系数显著增高（P〈O．05），组织切片显示暴露造成肝肿大和脂肪化发生。染毒组大鼠血清ALT、ALP和AST均显著升高（P〈O．05）；Hyp和LN在PFOS高剂量暴露组显著增高（P〈O．05）。染毒组大鼠肝细胞中MAO和GSH水平高于对照组，而SDH和SOD活力较对照组显著下降（P〈O．05）。结论PFOS暴露可能通过诱发氧化应激造成大鼠肝损伤。

英文摘要：

Objective To study the effect of perfluorooctane sulfonate （PFOS） exposure on rat liver. Meth- ods Rats were exposed with 0 mg/kg, 4 mg/kg and 8 mg/kg PFOS food for 3 months. Rats were sacri- ficed and PFOS concentration was determined by HPLC-LC/MS, histological changes of the liver tissue were examined as well. The contents including ALT, AST, ALP, LN and Hyp in serum were determined by ELISA kits. Besides, MAO, SOD, GSH and SDH were detected by the colorimetrie method. Results It showed that PFOS concentration increased after exposure and the ration of PFOSliver/serum was （4.22 ~0.99） and （3.33~0.75） respectively （P ~0.05）. Furthermore, PFOS exposure resulted in a significant hepatic injury with cell edema and diffuse steatosis in both groups （P ~0.05）. Compared with the control, ALT, ALP and AST increased significantly, especially in the high close group （P ~0.05）. Additionally, Hyp and LN, the indicators of liver fibrosis, significantly increased （P ~0.05）. Moreover, PFOS exposure increased the levels of MDA and GSH while reduced the levels of SDH and SOD （P （0.05）. Conclusion There was a significant injury effect with rat liver after PFOS exposure. The mechanism of action is related to oxidative stress.