中文摘要：

[目的]探讨孕哺期全氟辛烷磺酸（PFOS）暴露对子代大鼠糖代谢的影响。[方法]将Wistar孕鼠自孕0天（GD0）随机分为对照组（0 mg/kg）、低剂量组（0.6 mg/kg）和高剂量组（2 mg/kg）,每组10只;PFOS灌胃染毒至仔鼠出生后21天（PND21）断乳为止。采用高效液相/质谱法检测PND0、PND21时仔鼠血清PFOS含量;观察仔鼠体重变化趋势;比较9周龄和15周龄仔鼠空腹血糖、空腹胰岛素水平;检测仔鼠瘦素和抵抗素基因表达水平变化。[结果]PND21时低剂量组和高剂量组仔鼠血清中PFOS浓度分别为（16.00±1.27）mg/L和（80.54±6.55）mg/L（P〈0.05）。低剂量组雌性仔鼠出生后8、9周龄和高剂量7~9周龄,低剂量组雄性仔鼠出生8~12周龄、高剂量7、8、10周龄的体重均明显低于对照组（均P〈0.05）。高剂量组9周龄和低剂量组15周龄雌性仔鼠的胰岛素水平分别为（10.85±1.37）m U/L和（13.62±1.87）m U/L,均高于对照组（P〈0.05）;高剂量组9周龄雄性仔鼠的空腹血糖和胰岛素水平分别为（5.43±0.77）mmol/L和（13.23±1.81）m U/L,15周龄雄性仔鼠低剂量组分别为（4.99±0.54）mmol/L和（13.57±1.22）m U/L,15周龄高剂量组分别为（5.71±0.56）mmol/L和（13.44±2.97）m U/L,均高于对照组（P〈0.05）。高剂量组15周龄雄性仔鼠的抵抗素基因表达上调1.25±0.03（P〈0.05）,瘦素基因表达下调0.67±0.08（P〈0.05）。[结论]PFOS孕哺期暴露可能引起子代大鼠糖代谢异常,增加糖尿病患病风险。

英文摘要：

[ Objective ] To examine effects of maternal exposure to porfluorooctane sulfonate （PFOS） during pregnancy and lactation on glucose metabolism of rats offspring. [ Methods ] Pregnant Wistar rats were exposed to PFOS with doses of 0 （control）, 0.6 （low）, and 2 （high）mg/kg PFOS through gavage from gestation day 0 （GD0） to postnatal day 21 （PND21）. We applied high performance liquid chromatography-mass spectrometry to detect serum PFOS concentrations on PND0 and PND21; analyzed the trend of offspring＇s body weight variation; compared fasting blood glucose and fasting serum insulin levels of offspring at 9 weeks and 15 weeks; measured the expression of leptin and resistin genes. [ Results ] The serum PFOS concentrations increased to （16.00 ± 1.27）mg/L in the lowdose group and （80.54 ＋± 6.55）mg/L in the high-dose group on PND21 （P〈O.05）, respectively. Compared with the control group, the body weight was significantly lower in female offspring at 8-9 weeks in the low-dose group and 7-9 weeks in the high-dose group, and similar tendency was observed in the male offspring at 8-12 weeks in the low-dose group and at 7, 8, 10 weeks in the high-dose group （all P 〈 0.05）. The serum insulin of female offspring was （10.85 ± 1.37）mU/L at 9 weeks of the low-dose group and （13.62 ± 1.87）mU/L at 15 weeks of the high-dose group, both higher than that of the control group （P〈0.05）. For the male offspring of the high-dose group at 9 weeks, the fasting plasma glucose and the serum insulin were （5.43 ± 0.77）mmol/L and （13.23 ± 1.81）mU/L respectively; for the male offpring of the low-dose group at 15 weeks were （4.99 ± 0.54）mmol/L and （13.57 ± 1.22）mU/L respectively; for the male offspring of the high-dose group at 15 weeks were （5.71 ±0.56）mmol/L and （13.44 ± 2.97）mU/L respectively, all higher than those of the control offspring （P 〈 0.05）. Gene expression of glucose metabolism-related adipokine resistin were significantly up-regulated