中文摘要：

目的：研究hTERT和HIF启动子双调控的溶瘤腺病毒能否感染鼠膀胱癌细胞并表达治疗基因,为动物实验提供依据。方法：以不同浓度溶瘤腺病毒感染鼠膀胱癌细胞,生物倒置显微镜下动态观察细胞形态变化,RT-PCR检测SEA在细胞内mRNA表达,Western blot检测SEA蛋白表达。结果：镜下可见细胞感染腺病毒并最终裂解,RT-PCR和Western blot分别检测到实验组mRNA和蛋白的表达。结论：携带SEA基因的hTERT/HIF双调控溶瘤腺病毒可在鼠膀胱癌细胞内复制增殖并表达SEA基因,可用于治疗鼠膀胱癌的动物实验研究。

英文摘要：

Objective：To study human telomerase reverse transcriptase（hTERT）/hypoxia-inducible factor（HIF）-promoter regulated adenovirus whether can infect the mouse bladder cancer and express theoretical gene,providing evidence for the animal experiment.Methods：The cells of the mouse bladder cancer were infected with different concentration of adenovirus;The morphologic changes of cells were observed under the microscope;Staphylococcal enterotoxins A（SEA） in the nucleic acid expressions in tumor cells were detected by real-time quatiative polymerase chain reaction（RT-PCR） and protein expressions of SEA were detected by Western blot.Results：The cells were infected by adenovirus and disrupted,finally.The mRNA and protein of SEA were successfully expressed.Conclusion：The adenovirus can replicate and proliferate in the mouse bladder cancer cells and can be used in treating the mouse bladder cancer in vivo.