中文摘要：

目的 构建生物衍生型移植体GP-DPB，评价GP-DPB与转基因细胞复合后的体内骨修复能力，通过对不同移植方式的对比研究，探讨骨引导、骨传导和骨诱导3种骨再生机制结合下的组织工程成骨效能，以期实现对组织工程方法的优选。方法 体外扩增培养兔骨髓基质细胞（BMSCs），以脂质体介导转化生长因子（TGF-β1）基因转染BMSCs。取新西兰兔30只，建立兔桡骨中段1.5cm骨缺损模型，随机分为5组。第1组（TGF-β1-BMSCs／GP—DPB）为实验组，以GP—DPB与转基因细胞TGF-β1-BMSCs复合，第2组（TGF-β1-BMSCs／G-DPB）．第3组（TGF-β1-BMSCs／DPB）和第4组（BMSCs／DPB）为移植对照组，第5组为空白对照组，分别检测各移植体的成骨能力。结果观察骨缺损模型中各组骨修复效果，发现第1组（移植实验组，TGF-β1-BMSCs／GP—DPB）获得最优的修复。软X线定量、骨密度测量和生物力学测定均反映出，第1组的成骨速率和成骨质量明显优于其他各组，差异有统计学意义（P〈0．05）。结论 生物衍生型复合移植体GP-DPB与转基因细胞TGF-β1-BMSC复合后，能很好地结合骨传导、骨诱导和骨引导3种成骨机制，使生物材料、种子细胞和生长因子协调高效地发挥作用，充分体现了组织工程化骨中这种新移植模式的优势。

英文摘要：

Objective To develop a tissue-engineered bone graft modal, Methods The cultured bone marrow stromal cells （BMSCs） were transfected with the complexes of pcDNA3-TGF-β1 and Lipo- fectamine Reagent in vitro. Radial bone defect from 30 adult rabbits were divided into 5 groups （ n = 6 in each group）. Group 1 （TGF-β1-BMSCs/GP-DPB） served as experimental group, group 2 （TGF-β1-BM- SCs/G-DPB）, group 3 （TGF-β1-BMSCs/DPB） and group 4 （BMSCs/DPB） as transplant control group, and group 5 as blank control group. Osteogenic potential was detected in 5 groups espectively. Results All indexes revealed that the osseous regeneration was most excellent in group 1, including newly-formed bone tissue＇ s quality, as well as the velocity of bone repair. Conclusion With the natural derived implant GP-DPB combined with BMSCs earring TGF-β1 gene, we can acquire coordinated and effective osseous repair combining osteoconduction, osteoinduction and guided bone regeneration, so establish a valuable model to bone formation in tissue-engineering studies.