中文摘要：

目的评价以整合素为靶向的新型非病毒载体系统——含RGD肽基因导入系统K16GRGDSPC（记作K16-RGD）作为基因转染载体的可行性。方法固相合成法合成K16-RGD，以其为载体与不同比例的增强型绿色荧光蛋白质粒（pEGFP）混合转染兔骨髓基质干细胞（BMSCs），72h后用荧光显微镜观察转染效率及瞬时表达情况。结果转染效率与K16-RGD和质粒的比例有关，转染体系中K16-RGD（μg）：pEGFP（ μg）为3：1时EGFP有最高的表达效率。结论含RGD肽K16-RGD能有效介导外源基因在BM-SCs内的转染和表达，为下一步利用K16-RGD作为载体构建载基因仿生基质材料进行骨缺损修复研究奠定了基础。

英文摘要：

Objective To evaluate the feasibihty of using an integrin-targeted nonviral gene vector, RGD- containing peptide K16GRGDSPC, to mediate exogenous gene transfection into rabbit bone marrow stromal cells （BMSCs）. Methods The peptide was synthesized on a solid-phase batch peptide synthesizer. The cultured 3rd- genera-tion BMSCs were transfected with the complexes of pcDNA3-EGFP（pEGFP） and peptide vector at various ratio in vitro. Fluorescent microscope was performed to examine EGFP gene transfection and expression. Results The gene transfection efficiency was related with the proportion of K16-RGD to pEGFP plasmid and when the proportion was 1 to 3 in mass the highest value was obtained. Conclusions RGD-containing peptide K16GRGDSPC can be used as a new kind of nonviral gene vector. These results provided experimental foundations for next gene-modified bone tissue engineering study.