目的探讨呼吸道合胞病毒RNA(RSV-RNA)及呼吸道合胞病毒IgM(RSV-IgM)在小儿呼吸道感染诊断中的应用价值。方法选取2015年9月至2017年4月确诊呼吸道合胞病毒感染患儿56例,收集其咽部分泌物标本,对RSV-RNA进行检测;另抽取患儿空腹静脉血,对RSV-IgM进行检测,对两者的阳性检出率及与年龄、发病时间的相关性进行统计学分析。结果 RSV-RNA、RSV-IgM阳性检出率分别为89.29%、32.14%,RSV-RNA的阳性检出率显著高于RSV-IgM,差异有统计学意义(P〈0.05)。≤6个月,〉6个月至1岁患儿的RSV-RNA阳性检出率均高于RSV-IgM(P〈0.05);发病时间≤7d的患儿RSVRNA的阳性检出率高于RSV-IgM(P〈0.05)。结论在小儿RSV感染检测中,检测RNA比IgM具有更高的准确性,尤其是对年龄≤1岁、发病时间较短(≤7d)的患儿,早期诊断具有重要指导作用。
Objective To explore the application value of respiratory syncytial virus RNA(RSV-RNA)and respiratory syncytial virus IgM(RSV-IgM)antibody in the diagnosis of children with respiratory infection.Methods From September 2015 to April2017,56 cases of respiratory syncytial virus infection were selected,and the specimens of pharyngeal secretions were collected,and RSV-RNA was detected,in addition,fasting venous blood was taken from children,and the RSV-IgM antibody was detected.The positive rate of detection and correlation with age and time of onset were statistically analyzed.Results The positive rates of RSVRNA and RSV-IgM were 89.29% and 32.14%,respectively.The detection rate of RSV-RNA was significantly higher than that of RSV-IgM,and the difference was statistically significant (P〈0.05).The two groups,≤6 months and from 〉6 months to 1 year old,the detection rates of RSV-RNA in children was higher than RSV-IgM(P〈0.05),and the detection rate of RSV-RNA in children with ≤7 days of onset was higher than that of RSV-IgM (P〈0.05).Conclusion The detection of children with RSV infection,which the detection of RNA has higher accuracy than IgM,especially for children ≤1 year old,the onset time is shorter(≤7 days),plays an important guiding role for early diagnosis.