中文摘要：

本研究旨在观察替来他明麻醉后大鼠中枢神经系统c-fos基因的表达,了解替来他明在中枢神经系统的作用部位,探讨替来他明对中枢神经系统的作用机制。72只SD大鼠,随机分为生理盐水组、给药后10、30、60、90、100、120和180 min组,每组9只。腹腔注射替来他明50 mg.kg-1后,分别于给药前（生理盐水组）和给药后10、30、60、90、100、120和180 min经4%多聚甲醛（0.1 mol.L-1,PB,pH7.4）灌注后,取脑,将脑分为大脑皮层、海马、丘脑、小脑、脑干5个脑区,置于20%蔗糖溶液中24 h（4℃）脱水。冰冻切片,片厚10μm,按照Elivision法进行免疫组化染色,观察鉴别Fos阳性神经元并做阳性神经元计数。结查显示,对照组中仅发现少量Fos阳性神经元,试验组中Fos阳性神经元在大脑皮层、海马、丘脑、小脑、脑干内都有表达。替来他明腹腔注射10 min后Fos阳性神经元表达开始增加,60 min表达至高峰,90 min表达下降,180 min下降至基线水平,与给药前相比无显著性差异（P〉0.05）。结果提示,替来他明能诱导大鼠大脑皮层、海马、丘脑、小脑、脑干c-fos基因的表达,大脑皮层、海马、丘脑、小脑和脑干是替来他明的作用位点。

英文摘要：

To investigate the distribution of c-fos oncogene expression in rats central nervous system following tiletamine anesthesia.Tiletamine action sites in CNS were studied,and general anesthetic mechanisms were also discussed.72 SD rats were randomly divided into 8 groups.At 0,10,30,60,90,100,120 and 180 min after given tiletamine 50 mg·kg^-1 intraperitoneally,rats were perfused with 4% Paraformaldehyde（0.1 mol·L^-1,PB,pH 7.4）.The whole brain was separated into cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.Then these brain tissues were dehydrated 24 h by 20% sucrose（4 ℃）.Cryosections were 10 μm and Elivision Immunohistochemical technique for Fos-protein was used to observe and count on the Fos-like immunoreactive neurons.In normal saline group,there was few Fos-like immunoreactive neurons appeared.And Fos-like immunoreactive neurons were observed in cerebral cortex,hippocampus,thalamus,cerebellum and brain stem in experimental groups.Fos-like immunoreactive neurons expression increased at 10 min after tiletamine administrated by intraperitoneal injection,peaked at 60 min and decreased at 90 min.At 180 min after injection,Fos-like immunoreactive neurons recovered to base level,which had no significant difference compared with that before given tiletamine（P〉0.05）.The results showed that tiletamine induce expression of c-fos oncogene in the rats′ cerebral cortex,hippocampus,thalamus,cerebellum and brain stem.And the cerebral cortex,hippocampus,thalamus,cerebellum and brain stem at the action sites of tiletamine.