为探究奶牛酮病治疗对机体总抗氧化能力的影响,本研究选取南宁市某规模化养殖场的65头围产期奶牛作为研究对象,经血浆酮体定量检测,从中选取5头健康牛作为健康对照组,将检出的10头酮病患牛分为治疗组(5头)和阳性对照组(5头)。试验期间,对治疗组进行药物治疗,阳性对照组和健康对照组不做任何治疗。对各组奶牛分别于治疗前和停药后清晨空腹颈静脉采血,测定血浆酮体(改良水杨醛比色法)、血糖(氧化酶-过氧化物酶法)和TAC(铁还原比色法)水平。结果显示,治疗后,治疗组奶牛的血酮含量较治疗前极显著下降,血糖和总抗氧化能力含量极显著升高。阳性对照组和健康对照组的血酮和血糖含量、总抗氧化能力治疗前后变化不大,差异均不显著。结果表明,酮病治疗可提高奶牛血浆TAC水平,提示TAC的提高与酮病痊愈有关。
The purpose of this study was to explore the effect of ketosis treatment on plasma total antioxidant capacity (TAC) in dairy cows. In this study, 65 Holstein cows in peripartum period were chosen which came fi'om a dairy farm in Guangxi Nanning. After determining plasma ketone body(KET) level, five cows with normal KET was allocated as healthy control, while 10 cows with ketosis divided into treatment group (n=5) and positive control (n=5). During the test, the treatment cows were administrated with medicine and positive controls and healthy controls remained no treats. Blood samples were collected before and after test to determine the plasma levels of TAC, glucose and KET. The results showed that KET level decreased significantly (P〈0.05), the levels of glucose and TAC increased significantly (P〈0.05) after test in treatment cows. There were no significant differences at ketone, glucose and TAC levels between before and after test in both positive and healthy controls. Conclusion: Ketosis treatment can increase the plasma TAC level in dairy cows, which suggests the increase of TAC may associated with ketosis recovery.