中文摘要：

香烟吸烟与更低的精液质量被联系，但是香烟吸烟怎么改变精液质量，仍然保持不清楚。这研究的目的是屏蔽在有到香烟的每日的暴露的老鼠的精子的表示蛋白质吸的差别。2D 胶化电气泳动(2DE ) 和集体 spectrometry (MS ) 分析显示出的结果老鼠精子蛋白质侧面被香烟吸烟改变。并且对应于表示的差别的 22 最丰富的蛋白质在精子的 2DE 胶化看到样品被识别。这些蛋白质基于他们的功能被分类进不同的组，例如精力新陈代谢，复制，和结构的分子。而且，五蛋白质(Aldoa， ATP5a1， Gpx4， C，和 Spatc1 ) 的 2DE 和 MS 结果被西方的污点分析和反向的 transcriptase 聚合酶链反应验证。结果证明除了 Spatc1，另外的四蛋白质显示出在吸烟的组和控制组之间的统计上重要的不同蛋白质层次(P < 0.05 ) 。三基因(Aldoa， Gpx4，和 Spatc1 ) 的表达式是显著地不同的(P < 0.05 ) 在在吸烟的组和控制组之间的抄写水平。另外，在从 30 个男吸烟者和 30 个不抽烟的人的人的精子样品的五蛋白质(Aldoa， ATP5a1， Spatc1， C，和 Gpx4 ) 被西方的污点分析检测。与精力生产被联系的二蛋白质(Aldoa 和 C ) 被发现显著地被改变，建议这些蛋白质可以是为吸在精子的风险的评估的潜在的诊断标记。这些蛋白质的进一步的学习可以提供卓见进在吸人位于不孕下面的病原的机制。

英文摘要：

Cigarette smoking is associated with lower semen quality, but how cigarette smoking changes the semen quality remains unclear. The aim of this study was to screen the differentially expressed proteins in the sperm of mice with daily exposure to cigarette smoke. The 2D gel electrophoresis （2DE） and mass spectrometry （MS） analyses results showed that the mouse sperm protein profile was altered by cigarette smoking. And 22 of the most abundant proteins that correspond to differentially expressed spots in 2DE gels of the sperm samples were identified. These proteins were classified into different groups based on their functions, such as energy metabolism, reproduction, and struc- tural molecules. Furthermore, the 2DE and MS results of five proteins （Aldoa, ATP5al, Gpx4, Cs, and Spatcl） were validated by western blot analysis and reverse transcriptase-polymerase chain reac- tion. Results showed that except Spatcl the other four proteins showed statistically significant differ- ent protein levels between the smoking group and the control group （P〈 0.05）. The expressions of three genes （Aldoa, Gpx4, and Spatcl） were significantly different （P〈 0.05） at transcription level between the smoking group and the control group. In addition, five proteins （Aldoa, ATP5al, Spatcl, Cs, and Gpx4） in human sperm samples from 30 male smokers and 30 non-smokers were detected by western blot analysis. Two proteins （Aldoa and Cs） that are associated with energy production were found to be significantly altered, suggesting that these proteins may be potential diagnostic markers for evaluation of smoking risk in sperm. Further study of these proteins may provide insight into the pathogenic mechanisms underlying infertility in smoking persons.