中文摘要：

目的：评价可注射纳米羟基磷灰石/半水硫酸钙（n HAC/CSH）植骨材料的细胞相容性。方法：犬外周血基质细胞（d PBSCs）从健康成年犬的外周血中分离得到,通过直接接触或浸提液法检测n HAC/CSH的细胞相容性。制备n HAC/CSH的浸提液,通过MTT法检测细胞的增殖,采用流式细胞仪评价材料对细胞凋亡的影响,n HAC/CSH与d PBSCs共培养后光镜和扫描电镜下观察细胞形态。结果：随着培养时间的延长,对照组和浸提液组细胞数量不断增加,3天时两组细胞的凋亡比率相近,n HAC/CSH和d PBSCs共培养时,细胞伸展良好。结论：n HAC/CSH具有良好的细胞相容性。

英文摘要：

Objective： The aim of the study was to assess the cell biocompatibility of injectable nano-hydroxyapatite/collagen/calcium sulphate hemihydrate（n HAC/CSH） bone cement. Methods： Dog peripheral blood stromal cells（d PBSCs） were isolated from peripheral blood of healthy adult dogs. An in vitro study on the cytocompatability of n HAC/CSH was conducted. The d BMPCs morphology, proliferation and apoptosis assays were conducted using the direct contact and extraction method by microscopic observation, MTT analysis and flow cytometric method. Results： The MTT assay showed that proliferation proceeded in two groups. There was a spontaneous apoptosis of d BMPCs, with similar apoptosis rates on Day 3 in both the control culture and the extract group. Cells grew with n HAC/CSH cement readily attached to it and exhibited an elongated appearance. Conclusion： Studies in vitro revealed that the n HAC/CSH cement has good cell biocompatibility.