中文摘要：

目的通过短期和长期两组干预方式探讨维生素D对自发性高血压大鼠血压及肾素-血管紧张素系统（RAS）的影响。方法自发性高血压大鼠20只分成两组,短期干预组（S）和长期干预组（L）各10只。短期干预实验组（SE）5只,给予维生素D 3μg.kg-1.d-1溶于100μL丙二醇中腹腔注射,连续注射7d,观察3w,共计4w;短期干预对照组（SC）5只,给予单纯的丙二醇100μL腹腔注射7d,同SE组。长期干预实验组（LE）5只,给予维生素D 3μg.kg-1.d-1溶于100μL丙二醇中腹腔注射,每周2次,共计12w;长期干预对照组（LC）5只,给予单纯的丙二醇100μL腹腔注射,同LE组。药物干预同时观察两组大鼠的血压变化。分别在4w后和12w后分批处死大鼠,收集血清和心、肾、血管等组织。酶联免疫法（ELISA）检测血清中25（OH）D3、血钙、肾素、血管紧张素Ⅱ和醛固酮的浓度。心、肾、血管等做病理切片,HE染色。结果短期维生素D干预对大鼠的血压及RAS各指标影响不大。长期维生素D干预后,LE组大鼠血压明显低于LC组（P〈0.05）。酶联免疫检测结果显示LE组大鼠的25（OH）D3、血钙水平高于LC组（P〈0.05）,肾素、血管紧张素Ⅱ、醛固酮的浓度低于LC组（P〈0.05）。HE染色结果LE组高血压损害明显轻于LC组,LC组有明显高血压病理表现。结论长期规律的维生素D干预能够抑制RAS系统,降低血压,抑制或逆转高血压病理改变。

英文摘要：

Objective To explore the influence of vitamin D intervention on renin-angiotensin system （RAS） and blood pressure in spontaneously hypertensive rats by short-term and long-term vitamin D intervention. Methods Twenty spontaneously hypertensive rats were randomly divided into short-term inter vention group （S） and long-term intervention group （L）, each group had 10 rats. Short-term intervention experimental group （SE, 5 rats） was given vitamin D 3 μg.kg-1.d-1 dissolved in 100 μL propylene glycol by intraperitoneal injection for one week, and short-term intervention control group （SC, 5 rats） was given the equal volume of propylene glycol alone. Long-term intervention experimental group （LE, 5 rats） was received vitamin D preparation 3μg.kg-1.d-1 dissolved in 100μL propylene glycol by intraperitone- al injection （twice a week） for 12 weeks; Long-term intervention control group （LC, 5 rats） was given the equal volume of propylene glycol alone. Monitored the blood pressure of rats during the experiment every week. Rats were respectively executed at 4 weeks later and 12 weeks later. Serum and organization were collected. Serum 25（OH）D3, calcium, renin, angiotensin II and aldosterone concentration were detected by enzyme-linked immunosorbent assay （ELISA）. Pathological changes were examined by HE staining. Results The impact of short-term vitamin D intervention on blood pressure and RAS in spontaneously hypertensive rats was not significant. After long-term vitamin D intervention,the blood pressure of LE group was lower than that in LC group （P 〈0.05）. Serum 25（OH）D3, calcium concentration in LE group were higher than that in LC group （P 〈0.05）, Renin, angiotensin II , aldosterone concentration were lower those in LC group （P〈0.05）. HE staining displayed there were hypertensive pathological changes in LC group, but which was not obvious in LE group. Conclusion Long-term vitamin D intervention could inhibit RAS, lower blood pressure, inhibit or reverse hyp