中文摘要：

目的：探讨上皮性卵巢癌（EOC）患者外周血、卵巢癌组织和癌旁腹膜中Treg / Th17是否存在失衡。方法：选取2011年9月至2013年12月同济大学第一妇婴保健院收治的16例 EOC（EOC 组）、11例良性上皮性肿瘤（良性肿瘤组）及14例健康成年女性（对照组）,收集患者外周血并分离淋巴细胞；收集4例 EOC 患者腹水并分离淋巴细胞。流式细胞术检测外周血及腹水中 Th17、Treg 细胞占 CD4＋T 细胞的比例。选取同一时间段在本院手术患者术中留取的卵巢肿瘤组织、腹膜组织及转移灶组织,包括13例 EOC、16例 EOC 种植灶癌旁腹膜、5例良性卵巢肿瘤组织及腹膜,用免疫荧光染色分析 Th17及Treg 细胞在良性卵巢肿瘤、EOC 原发灶和癌旁腹膜中的浸润情况。结果：（1）EOC 患者外周血中 Treg 比例为（5.16±3.85）%,显著高于对照组[（2.41±1.76）%]）和良性肿瘤组[（2.3873±2.336）%]（P =0.025,P =0.043）,后两组比较无统计学差异。 EOC 患者外周血中 Th17细胞比例为（3.15±3.045）%,显著高于对照组[（1.22±1.13）%]（P =0.044）；良性肿瘤患者[（1.93±1.745）%]与 EOC 患者和对照组比较,差异均无统计学意义；Treg / Th17细胞比值在3组间均无统计学差异。 EOC 患者腹水与外周血中的 Th17、Treg细胞比例及 Treg / Th17比值比较,差异均无统计学意义（P〉0.05）。（2）EOC 组肿瘤组织中 Treg 和 Th17细胞比例及 Treg / Th17比值分别为（0.1062±0.077）%、（0.143±0.056）%和0.80±0.56,与良性肿瘤组织[0%、（0.0789±0.11）%、0]比较,均显著增高（ P 均〈0.05）。（3）卵巢良性肿瘤腹膜中未见 Treg 及 Th17细胞浸润,EOC 腹膜种植灶癌旁腹膜中 Treg、Th17细胞比例及 Treg / Th17比值分别为（0.1024±0.1）%、（0.2254±0.23）%和0.8113±1.097,较良性肿瘤组均显著提高（P〈0.05）。（4）早期和晚期 EOC 组织中 Th17、Treg 比例以及 Treg / Th17比值比较,差异均无统

英文摘要：

Objective：To investigate the balance of Treg / Th17 in the peripheral blood, tumor tissue and peritoneum of epithelial ovarian cancer. Methods：We collected the peripheral blood from 14 healthy donors（control group）,11 benign ovarian tumor patient（benign group） and 16 EOC patients（EOC group） and also collected 4 cases ascites from EOC patients during Sep. 2011 to Dec. 2013 in Shanghai First Maternity and Infant Hospital. Lymphocyte in periph-eral blood and ascites were isolated,and we used Flow cytometry to detect the rate of Th17 cell and Treg cell in CD4＋ T lymphocyte. Collect the tissue samples after the operation during the same period,including EOC tissues （13 cases）,peritoneum tissue adjacent to carcinoma （16 cases）,benign tumor tissues and normal peritoneum （5 cases）,The infiltration of Th17 cell and Treg cell in tumor tissue and peritoneum were detected by immunofluorescence. Results：（1） The frequency of Treg cell and Th17 cell in EOC patients[（5. 16±3. 85）% ] was significantly higher than that in healthy donors[（2. 41 ±1. 76）% ] （P = 0. 025） and patients with benign tumors[（2. 3873±2. 336）% ] （P = 0. 043）. There was no significance difference between the patients with benign tumors and healthy donors. The frequency of Th17 cell in EOC patients[（3. 15±3. 045）% ]was significantly higher than that in healthy donors[（1. 22±1. 13）% ]（P= 0. 044）. There was no significance difference between the patients with benign tumors[（1. 93±1. 745）% ] and EOC patients. Neither was between the patients with benign tumors and healthy donors. Ratio of Treg / Th17 cell in three gorups shows no significance difference. （2） Compared with the patient with benign tumors[Treg cell 0% ,Th17 cell 0. 789% ,Treg / Th17 0% ],the expression of those two cells was significant higher in EOC patients＇ ovarian tumor（Treg cells was[（0. 1062±0. 077）% ],Th17 was[（ 0. 143±0. 056）% ], the ratio of Treg /Th17 is 0. 80±0. 56 ） （P〈0. 05）. （3）