中文摘要：

目的应用双向凝胶电泳（Two-dimensional gel electrophoresis,2-DE）方法分析烟染毒大鼠外周血淋巴细胞蛋白质组表达的改变。方法雄性Wistar大鼠随机分正常对照组和烟染毒组,烟染毒组分小、中、大剂量,分离各组大鼠的外周血淋巴细胞,提取总蛋白,2-DE分离总蛋白,电泳凝胶银染显色,用ImageMaster 2D Platinum软件对获得的蛋白图谱加以分析,寻找差异蛋白点。结果与正常对照组大鼠图谱比较,烟染毒组图谱中与剂量有相关性变化的差异表达蛋白点有10个,其中6个随烟染毒剂量增加表达下降,4个随烟染毒剂量增加表达增强。结论2-DE可以有效分离大鼠外周血淋巴细胞蛋白质,大鼠烟染毒后淋巴细胞蛋白质的表达发生改变。

英文摘要：

Objective To analyze the change of lymphocytes proteome in rats exposed to cigarette smoke by two-dimensional gel electrophoresis（2-DE）.Methods Male wistar rats were randomly divided into four groups： the control group and the smoking groups.The peripheral blood lymphocytes of rats were isolated and the total proteins were extracted and separated by 2-DE.Then the gels were stained with silver and the protein spots were analyzed with ImageMaster 2D Platinum software.Results Compared with the control, six protein spots expressed obviously descending and four spots expressed obviously increased in a dose-dependent manner in the smoking groups.Conclusion 2-DE is effective to separate lymphocytes proteins and the results indicate that the protein profiles between the smoking groups and the control are different.