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灯台树内生放线菌多样性及抗菌活性评价
  • ISSN号：0253-2654
  • 期刊名称：微生物学通报
  • 时间：2011
  • 页码：780-785
  • 分类：Q523[生物学—生物化学]
  • 作者机构：[1]云南大学云南省微生物研究所云南省生物资源保护与利用重点实验室,云南昆明650091, [2]中国科学院南海海洋研究所广东省海洋药物重点实验室,广东广州510301
  • 相关基金：NSFC-云南联合基金项目（No.U0932601）
  • 相关项目：从云南特境放线菌新物种中发现抗癌新化合物及作用机制研究
作者： 黄海玉|李洁|赵国振|朱文勇|段学伟|李秀萍|赵立兴|徐丽华|
关键词： 灯台树, 内生放线菌, 分离, 鉴定, 抗菌活性, Alstonia scholar, Endophytic actinomycetes, Isolation, Identifiation, Antimicrobial activity
中文摘要：

从云南西双版纳采集7份灯台树样品,经过表面消毒,用4种分离培养基分离得到105株内生放线菌。经16S rRNA基因序列分析鉴定,分布于7个科9个属。利用5种植物病原真菌指示菌对所有菌株的发酵液进行抗菌活性检测。结果显示,有12.4%、14.3%、11.4%、12.4%、8.6%的菌株分别对镰刀霉、疫霉、赤星霉、苹果炭疽、白色念珠菌有抗性。对3株具有广谱抗菌活性的菌株进行再次发酵和抗菌活性复筛,结果显示这3株的抗菌活性稳定,并可能含有生物碱类化合物。

英文摘要：

One hundred and five endophytic actinomycetes were isolated using four different media from surface-sterilized tissues of 7 plants samples of Alstonia scholar collected from Xishuangbanna Yunnan province,southeast China.The results showed that they belong to 9 genera,7 families by 16S rRNA gene sequences analysis.All actinomycete strains fermentation liquids were carried out the an-timicrobial activities test against five plant pathogenic fungi.The results showed that 12.4%,14.3%,11.4%,12.4%,8.6% endophytic actinomycetes presented antimicrobial activity for Fusarium graminearum,Phytophthora nicotianae,Alternaria alternata,Colletotrichum gloeosporioides and Candida albicans,respectively.Three strains with strong antibacterial activities were refermented and rescreened,their antimicrobial activities were stability,and may produce alkaloids.

同期刊论文项目
从云南特境放线菌新物种中发现抗癌新化合物及作用机制研究
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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编：赫荣乔
  • 地址：北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编：100101
  • 邮箱：xuj@sun.im.ac.cn
  • 电话：010-64807511
  • 国际标准刊号：ISSN：0253-2654
  • 国内统一刊号：ISSN：11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:29487