中文摘要：

运用蛋白质组学比较龙眼（Dimocarpus longan Lour.）正常成花和成花逆转花芽的蛋白质组变化,结果表明,ANS蛋白（anthocyanidin synthase）在龙眼成花逆转花芽中下调表达。应用RACE方法克隆ANS蛋白的全长cDNA,长度为1477 bp,包括1个1071 bp的开放阅读框,编码357 bp的氨基酸序列,GenBank的登录号为FJ479616（GI：218202927）。将ANS在大肠杆菌中表达,获得1个约46 kD的外源蛋白。这说明ANS蛋白在龙眼成花逆转过程中起作用。

英文摘要：

The changes in proteome of normal flower and floral reversion buds in longan（Dimocarpus longan Lour.） were studied by using Proteomics method.The results showed that ANS（anthocyanidin synthase） protein was found down-regulated expression in floral reversion buds.ANS cDNA was obtained（GenBank access number： FJ479616） by using RACE method,its full length was 1477 bp,with a 1071 bp open read frame.ANS gene was expressed in E.coli,and a 46 kD heterologous protein was obtained.It suggested that ANS protein play a role in floral reversion in longan.