中文摘要：

基于 bioactive polymerhydroxyapatite， composites 引起了严重限制他们的扩大申请的感染的骨头织物脚手架。在这研究，我们建议了一个实际离子替换方法在二水平的、三维的 chitosan/nano-hydroxyapatite 脚手架的表面上在 situ 银磷酸盐综合。在磷酸盐的银离子的版本测试缓冲了盐(PBS ) 溶液被执行证明银离子在学习的起始的 6days 期间连续地被免除银磷酸盐。与银硝酸盐答案的不同集中对待的脚手架的抗菌剂性质和 cytocompatibility 被估计由在有 Escherichia coli 和 MC3T3-E1 的 vitro 试金分别地。包含银的脚手架的能力被抑制地区(15mm ) 和高杀菌的率证实导致细菌抑制(>99%) 。房间增长，形态学和在有低银磷酸盐内容的支架上有教养的 MC3T3-E1 的碱的磷酸酶活动与在控制样品上有教养的那些是可比较的。

英文摘要：

Bone tissue scaffolds based on bioactive poly- mer-hydroxyapatite composites have caused infections that seriously limit their extended application. In this study, we proposed a practical ion substitution method to synthesize in situ silver phosphate on the surface of a two-level, threedimensional chitosan/nano-hydroxyapatite scaffold. A release test of silver ions in a phosphate buffered saline （PBS） solution was performed to demonstrate that silver ions were released continuously from the silver phosphate during the initial 6 days of the study. The antibacterial property and cytocompatibility of the scaffolds treated with different concentrations of silver nitrate solution were assessed by in vitro assays with Escherichia coli and MC3T3-E1, respectively. The ability of the silver-containing scaffolds to induce bacteriostasis was confirmed by the inhibition zone （15 mm） and high bactericidal rate （〉99 %）. Cell proliferation, morphology and the alkaline phosphatase activity of MC3T3-E1 cultured on the scaffold with low silver phosphate contents were comparable with those cultured on control samples.