近红外光谱技术是光谱测试技术、化学计量学技术与计算机技术的有机结合,文章立足于食品有效成分无损定量检测的目标,介绍了微型近红外光谱分析系统的研制过程。作为系统测试的基础,文章重点研究了适用于在线实时分析的微型化近红外光谱仪,研制出的微型近红外光谱仪样机工作波长:850~1690nm,分辨率小于10nm。通过对样机主要参数的测试表明,性能接近国外同类产品水平。另外,化学计量学与计算机技术的结合是系统的核心,提出了基于最小二乘的局部加权回归(LS-LWR)的建模方法。最后,应用研制出的系统对葡萄糖水溶液定量测试实验表明,其预测集的相关系数达到了0.995,预测标准偏差为0.06。
Near-infrared spectrometer is the integration of spectrum test technology, stoichiometry technology and computer technology. In the present paper, based on effective food ingredients and non-invasive quantitative detection, the development process of the micro-near-infrared spectrometer system was introduced. Spectrometer is the basis of the system. This paper focuses on the development of the micro-near-infrared spectrometer applicable to on-line real-time testing. A micro-near-infrared spectrometer prototype was developed successfully, its main technical parameter was tested, and the result shows: its operating wavelength is: 850-1 690 nm, optical resolution is: less than 10 um, and its performance has achieved the level of the congener foreign products. Stoichiometric technology and computer technology is the core of the system. LS-LWR modeling methods were proposed. Finally, the quantitative test for glucose water solution using the micro-near-infrared spectrometer shows that the correlation coefficient of prediction model is 0. 995, and the corresponding RMSEP is 0. 06.