本文基于一种阵列电极的低工作电压电泳芯片分离模型,在其中微管道的电场模拟的基础上,结合微流体动力学特性,以分离管道侧壁排布电极并等间距施加电压,建立电泳芯片低工作电压分离过程的流场模型,利用CoventorWare软件分析单组分和双组分试样在微分离管道中流场的模拟,发现组分在常规电压和低工作电压两种分离模式下,其迁移速度近似相等;对于双组分,分离电压可大大降低同时,还可保证原来的分离度,低电压电泳过程中,工作电压可降低至30V.证实了阵列电极和运动梯度场实现低电压电泳的可行性和有效性。
On the basis of the theory model of low voltage separation on CE chip with arrayed electrodes along both sides of the micro-channels, and electric fields simulating results in micro channel by ANSYS software and fluidic dynamics, solid model of low voltage electrophoresis with channel side arrayed electrodes was established. Low voltage was applied to arryed electrodes in the moving way with equal distance each time. CoventorWare software were used to simulate and calculate the fluidic moving in micro separating channel and the process of electrophoresis separation. Taking the signal and double components as samples in low voltage CE chip, the simulating results shown that component in the fluid moved at the almost same speed under the common CE and low voltage CE modes. While the good resolution was got under low voltage CE modes, the voltage could even decrease to 30 V. Furthermore, computer simulation results of the flow field had proved the possibility and practicality of the low voltage CE chip with side arrayed electrodes.