中文摘要：

目的研究分析喀什木纳格葡萄树伤流液中多糖的分离纯化及其单糖组成成分。方法采用醇沉法提取多糖，Sevage法脱蛋白，经DEAE-52纤维素及SephedexG-150凝胶柱分离纯化，采用气相色谱分析法测定单糖组成。结果80％乙醇沉淀、Sevage法除蛋白得粗多糖；葡萄树伤流液粗多糖经过DEAE52分离纯化分别得到GBSK-1、GBSK-2、GBSK-3、GBSK-4、GBSK-5、GBSK-6、GBSK-7和GBSK-8共8个多糖组分。其中4个多糖组分经过SephadexG-150纯化后，其洗脱峰均为单峰；通过GC分析得出GBSK-3的单糖组成较简单，主要由木糖组成；多糖GBSK-2、GBSK-5的单糖组成相似，主要由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖组成；多糖GBSK-1的单糖组成主要南鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖组成。结论该方法较好地分离纯化葡萄树伤流液多糖。GC分析适用于多糖中的单糖组成分析，为葡萄树伤流液的开发和利用提供了科学依据。

英文摘要：

Objective To separate and purify of Kaxigar munaka grapevine bleeding sap polysaccharides and analysis of monosaccharide composition. Methods Prepare crude polysaccharide using alcohol precipitation and by improved Sevage method. Purify the obtained crude polysaccharide by DEAE-52 and Sephadex G- 150 column chromatography. Analysis the monosaccharide composition by gas chromatography. Results To get crude polysaccharide by 80% alcohol precipitation and to remove proteins by Sevage method. The crude polysaccharide was separated by DEAE-52, and obtained 8 polysaccharides, respectively GBSK-1, GBSK-2, GBSK-3, GBSK-4,GBSK-5,GBSK-6,GBSK-7 and GBSK-8. After being purified, 4 polysaccha- rides eluting peaks were unimodal. From the result obtained by GC analysis, monosaccharide composition of GBSK 3 was relatively simple and mainly composed of xylose. The polysaccharides GBSK-2, GBSK-5 had similar monosaccharide composition and they were mainly composed of rhamnose, arabinose, man- nose, glucose and galactose. While the polysaecharides GBSK-1 was mainly composed of rhamnose, arabi- nose, xylose, mannose, glucose and galactose. Conclusion Results indicate that this method is suitable for the separation and purification of grapevine bleeding sap polysaccharide. It is suitable to analysis the monosaccharide composition of grapevine bleeding sap polysaccharides by gas chromatography. A scientific basis is provided for the development and utilization of grapevine bleeding sap.