中文摘要：

目的：研究异绿原酸A在大鼠体内的生物利用度和药代动力学,为该制剂的临床应用提供参考。方法：建立大鼠血浆中异绿原酸A的HPLC检测,检测波长300 nm,流动相甲醇-0.1%磷酸水（50∶50）。考察大鼠经静脉注射（32 mg·kg^-1）与灌胃（90 mg·kg^-1）给予异绿原酸A后的血药浓度变化,利用3P97软件计算药动学参数,根据药时曲线下面积AUC0-∞和给药剂量,计算异绿原酸A的绝对生物利用度。结果：异绿原酸A在0.16～110.00 mg·L^-1线性良好（R2=0.998）;质量浓度分别为0.43,6.88,55.00 mg·L^-1的异绿原酸A的提取回收率分别为（89.43±2.84）%,（93.16±3.95）%,（85.91±2.04）%;日内精密度RSD分别为11.8%,4.0%,4.0%,日间精密度RSD分别为6.5%,5.8%,5.8%。大鼠静脉注射和灌胃异绿原酸A后,异绿原酸A在大鼠体内的代谢过程均符合二室模型,消除半衰期分别为（29.49±0.75）,（44.48±0.13）min,AUC0-∞分别为（355.40±32.58）,（319.91±51.00）mg·min-1·L^-1。异绿原酸A在大鼠体内的绝对生物利用度30.71%。结论：异绿原酸A在大鼠体内的过程符合线性动力学过程,且代谢快、半衰期短。

英文摘要：

Objective： To study pharmacokinetics and bioavailability of isochlorogenic acid A in rats with different adiminstration way. Method： A HPLC method was developed for determination of isochlorogenic acid A in rat plasma. Intravenous and intragastric doses of 32 mg · kg^-1 and 90 mg · kg^-1 were respectively administered,the concentration of isochlorogenic acid A were determined with HPLC,detection wavelength was set at 300 nm. Pharmacokinetic parameters were calculated with 3P97 software. Absolute bioavailability of isochlorogenic acid A was calculated according to AUC0-∞and doses of isochlorogenic acid A by intravenous and intragastric administration. Result： Excellent linear relationship was obtained in the range of 0. 16-110. 00 mg·L^-1 with R2 of 0. 998. Recoveries of isochlorogenic acid A with three concentrations（ 0. 43,6. 88,55. 00 mg ·L^-1） were（ 89. 43 ± 2. 84） %,（ 93. 16 ± 3. 95） % and（ 85. 91 ± 2. 04） %. The intra-day RSD were 11. 8%,4. 0% and 4. 0%. The inter-day RSD were 6. 5%,5. 8% and 5. 8%, respectively. Metabolic processes of isochlorogenic acid A in rats fitted in with two-compartment model following intravenous and intragastric administration. Elimination half-life were（ 29. 49 ± 0. 75） min and（ 44. 48 ± 0. 13） min. AUC0-∞were（ 355. 40 ± 32. 58）,（ 319. 91 ± 51. 00） mg·min^-1·L^-1. Its absolute bioavailability in rats was 30. 71%.Conclusion： Metabolic processes of isochlorogenic acid A in rats conform to linear dynamics with fast metabolism and short half-life.