中文摘要：

目的：对大鼠原位肠肝血管灌流模型的灌流液组成成分进行优化,以获取最佳灌流液配方。方法：对灌流条件进行优化,分别对Krebs-Rin-ger（K-R）液加牛血清白蛋白和甘露醇、K-R液加牛血清白蛋白和右旋糖酐T-40二者进行对比,找出最佳的灌流介质。然后对最佳的灌流介质中牛血清白蛋白的含量进行了优化。结果：K-R液加牛血清白蛋白和右旋糖酐T-40组优于K-R液加牛血清白蛋白和甘露醇组;5%牛血清白蛋白的灌流液是一个较为经济理想的灌流液。结论：在灌流介质中5%牛血清白蛋白是一个较为理想和经济的比例。

英文摘要：

AIM： To optimize perfusate composition of in situ perfused rat intestine-liver model and achieve the best perfusate formula.METHODS： The perfusion conditions were optimized,respectively.The Krebs-Ringer NaHCO3 solution with bovine serum albumin and mannitol and the Krebs-Ringer NaHCO3 solution with bovine serum albumin and dextran two T40 were contrasted for finding out the best perfusate medium.Then bovine serum albumin content in the optimal perfusion medium was optimized.RESULTS：K-R solution with bovine serum albumin and dextran T40 group was better than K-R solution with bovine serum albumin and mannitol group;5% bovine serum albumin in perfusate is a more economic and ideal proportions.CONCLUSION： 5% bovine serum albumin in perfusate is a more economic and ideal proportions.